Hybridoma Workflow for Antibody Discovery
Hybridoma generation and screening of large antibody libraries
Hybridoma technology is a method for mass-producing antibodies in a hybrid cell line generated from the fusion of antibody-producing B-cells with an immortalized myeloma cell line, now called a hybridoma cell. Because every B-cell produces a unique antibody, single-cell cloning of hybridomas can be used to generate a diverse library of unique monoclonal antibodies at a large scale, which are very frequently used in the prevention, diagnosis, and treatment of disease.
In this video, Justin Dranschak, manager for BioPharma platforms, presents our solution for a hybridoma workflow and references the systems to aid in your research.
Hybridoma workflow
Step 1: Fusion
The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma.
Step 2: Clone screening
The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies.
Step 3: Cell growth
Cell growth is determined by monitoring cell divisions over a given period of time using label-free imaging.
Step 4: Specificity and cross reactivity
The process of analyzing the ability of an antibody to bind to only one target antigen.
Step 5: Binding affinity and internalization
Binding affinity is the strength of the binding interaction between a single biomolecule (e.g. protein or DNA) to its ligand/binding partner (e.g. drug or inhibitor). Internalization is the process of monitoring the ability of a given particle to enter into the cell.
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