SCIENTIFIC POSTER
Monitoring of the
T-cell invasion assay using a 3D spheroid model
Despite the boom of CAR T-cell therapies, the discovery of novel immunotherapies that specifically enhance T-cell response against cancer cells remains a challenge.
In response to the absence of robust, in vitro models to evaluate these immunotherapies throughout their development, we developed a workflow to easily acquire 3D and 4D T-cell datasets and analyze phenotypic evolution over time. In this application note, we describe this workcell for the generation of 3D tumor spheroids co-cultured with T-cells as a proof-of-concept model for CAR T assays. Further, we developed an image analysis approach that uses deep learning to accurately segment biological objects of interest and machine learning to quantify the phenotypic changes in the spheroids using only brightfield images.
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Product family
Imaging
Product primary application
ImageXpress Confocal HT.ai; ImageXpress Micro Confocal High-Content Imaging System; MetaXpress High-Content Image Acquisition and Analysis Software; IN Carta Image Analysis Software
CMP
701Rn00000GUcY9IAL
Title
Monitoring of the T-cell invasion assay using a 3D spheroid model
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