APPLICATION NOTE
Cell culture automation of the 3D cancer spheroid assay with the CellXpress.ai cell culture system
Finding efficient anti-cancer drugs and drug combinations is critical for therapy success.
Being highly representative of the structure and behavior of tumors, 3D cancer cell models, such as tumoroids, are highly valuable tools for cancer research and drug development. However, manual 3D cell culture workflows are time-consuming, error-prone, and inconsistent, making their adoption for high-throughput drug screening cumbersome.
To expedite and standardize the spheroid assay, we developed 3D cell culture automation methods using the CellXpress.ai™ Automated Cell Culture System to provide automated plating, passaging, media exchange, and organoid monitoring in response to compound treatment, and endpoint assays. In this app note, we describe the automation of a colorectal cell culture workflow where we automated the culture and imaging of colorectal cancer 3D spheroids formed from HCT116 cell lines in U-shape low attachment plates.
