APPLICATION NOTE
Automation of 3D intestinal organoids culture with CellXpress.ai Automated Cell Culture System
Attrition in the therapeutic pipeline can often be attributed to the lack of translational efficacy from the pre-clinical phase to the clinic.
Organoids show great promise as a game-changer in disease modeling and drug screening since they better resemble tissue structure and functionality and show more predictive responses to drugs. However, challenges associated with the practical adoption of organoids, such as assay complexity, reproducibility, and the ability to scale up have limited their widespread adoption as a primary screening method in drug discovery.
To alleviate the bottlenecks that come with labor-intensive manual protocols, we developed the CellXpress.ai™ Automated Cell Culture System to automate the entire organoid culture process for prolonged complex workflows. The CellXpress.ai system offers AI-driven, end-to-end cell culture management – from liquid handling to endpoint assay execution to complex image analysis. In this app note, we present results from an automated intestinal workflow which can be used as the basis for other commonly used 3D organoid protocols in matrix domes.
