Dated: Apr 13, 2023
Publication Name: The Royal Society of Chemistry
Citations : Selecting the appropriate indirect viability assay for 3D paper-based cultures: a data-driven study
Cellular viability measurements quantify decreased proliferation or increased cytotoxicity caused by drug candidates or potential environmental toxins. Direct viability measures count each cell to provide an accurate readout. This approach can prove analytically challenging and time-consuming when cells are maintained in 3D structures akin to tissues or solid tumors. While less labor-intensive, indirect viability measures can be less accurate due to the heterogeneous structural and chemical microenvironment that arises when cells are maintained in tissue-like architectures and in contact with extracellular matrices.
Contributors: Zachary R. Sitte, Tyler S. Larson, Julie C. McIntosh, Melanie Sinaniana and Matthew R. Lockett