Plating flexibility

HEK cells stably expressing human NET were plated overnight (open blue circles) or on the day of assay (filled black squares) at 20,000 cells per well in poly D-lysine-coated 384-well plates and allowed to adhere for 3 hours. Medium was removed and nomifensine in HBSS/0.1% BSA was incubated with cells for 10 minutes at 37°C prior to dye addition. The assay was read on the FlexStation® 3 Reader in kinetic mode for 30 minutes. The inhibition curve is expressed as area under the curve.