Nisoxetine inhibition in HEK-hSERT cells

HEK cells stably expressing human SERT were plated O/N at 10,000 cells per well in poly D-lysine-coated 384-well plates. Medium was removed and nisoxetine (published Ki = 383 nM) in HBSS/0.1% BSA was incubated with cells for 10 minutes at 37°C prior to dye addition. The assay was performed according to the Experimental Protocol and read on Molecular Devices' FlexStation® 3 Reader in kinetic mode for 30 minutes.