IC50 correlation for DAT, NET and SERT (customer results)

Cells stably expressing human DAT, NET and SERT were plated overnight at 60.000 cells/well (96-well plate) and treated with 8 different known inhibitors. After 10 minutes the Dye Solution was added and the assay was run according the protocol described above. The log Ki was calculated from the resulting data using the Cheng-Prusoff equation and a correlation with known Ki values for all three transporters was determined. The correlation coefficients R2 for each transporter is presented in the graph, the correlation coefficient (R2 value) over all 24 values was 0.91.