Fibroblasts vs. adipocytes; insulin dose-response

3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µl of DMEM/FBS in a 96-well plate and incubated at 37°C for 5 hours, then serum deprived for 1 hour. Different concentrations of insulin were added into the well and incubated for 30 minutes at 37°C, 5% CO2 incubator. At the end of the incubation time, 100 µl of fatty acid mixture was added into the well, and kinetic readings were started immediately with a FlexStation® 3 Reader. Traces A to F correspond to adipocytes with 160, 16, 8, 1.6, 0.16, and 0 nM insulin, respectively; traces G and H correspond to fibroblasts with 0 and 160 nM insulin, respectively.