Fibroblast vs. adipocytes

3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µl of DMEM/FBS in a 96-well plate and incubated at 37°C for 5 hours. Growth medium was then replaced with fatty acid uptake solution. Kinetic readings were started immediately with a Molecular Devices FlexStation® 3 Reader. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only).