cGMP Calibration Curve

Calibration curve, 96-well assay format. Calibration curve for the CatchPoint cyclic-GMP Kit. Data was taken 60 minutes after addition of Stoplight Red substrate. The EC50 of the cGMP calibration curve was 2.2 nM (264 fmol). Minimum detectable concentration was 0.2 nM (24 fmol).