Atrial Natriuretic Peptide Dose Response

Atrial Natriuretic peptide dose response in RFL-6 cells, 96-well assay format. A confluent monolayer of RFL-6 cells in a 12-well plate was pre-incubated for 10 minutes in Krebs Ringer bicarbonate buffer containing the non-selective phosphodiesterase inhibitor IBMX. Cells were subsequently stimulated with ANP for 15 minutes, lysed, and analyzed using the cGMP Fluorescent Assay Kit.