Agonism of the Muscarinic M1 Receptor on CHO M1 Cells

On the FlexStation® 3 Multi-Mode Microplate Reader, carbachol was used to stimulate the muscarinic M1 receptor on CHO M1 cells to generate concentration response curves (CRC), comparing Fura-2 QBT Dye to the BD Kit and the traditional Fura-2 wash protocol. EC50 values were similar for all methods, and were within range of published values.