Agonism of Endogenous H1 Receptor on HeLa Cells

Histamine was used to stimulate the endogenous histamine receptor on HeLa cells. Assay was measured using the FLIPR Tetra® High-Throughput Cellular Screening System with UV LEDs. Generated concentration response curves (CRC) comparing Fura-2 QBT Dye to the BD Ratiometric Calcium Kit and traditional Fura-2 wash protocol. EC50 values were within a half log while the Z-factor at EC80 was the largest with the Fura-2 QBT Calcium Kit.