Protein Detection Based on Europium Labeled Proteins

Western Blot Protein Detection and Quantitation Based on Europium Labeled Proteins using a Plate Reader

Here we report a novel system for western blot analysis that is incorporated into a SpectraMax® multi-mode microplate reader. Membranes are probed with europium labeled secondary antibodies or streptavidin that label the protein of interest. Europium (Eu) has a long fluorescence lifetime, on the order of 1 msec, and detection is done with time resolved fluorescence (TRF) detection mode, which significantly reduces background from auto-fluorescence or other sources of short lifetime emissions.