Protein and nucleic acid quantification (specific)
Many biological experiments aim to alter protein expression within a cell, whether through genetic engineering or drug treatment. In order to understand the effect of such manipulation, it is critical to measure the response of specific protein(s). Antibodies are commonly used in bioassays (immunoassays) for this purpose due to their inherent ability to specifically bind to a protein of interest.
Learn more about the common applications for protein and nucleic acid quantification (specific) to aid in your COVID-19 vaccine research:
- ELISA (Cytokine secretion) - Enzyme-Linked Immunosorbent Assay (ELISA)
- Western blot - Western Blot
- HTRF (cytokine secretion) - Normalize HTRF cytokine assays to cell viability
- AlphaScreen/AlphaLISA - AlphaLISA Screen on the SpectraMax Paradigm Multi-Mode Microplate Detection Platform
- ValitaTITER (IgG quantification using fluorescence polarization) - Streamline the workflow for measuring IgG in cell line development