Protein and nucleic acid quantification (non-specific)

Experimental protocols involve a significant amount of assay/sample preparation, however, they also lengthen timelines in order to assure quality. Balancing the need for speed with the need for quality assurance can be accomplished with non-specific methods of quantification. Total amounts of proteins and/or nucleic acids can be measured using UV absorbance on a microplate reader.

Here’s a few application notes on protein and nucleic acid quantification (non-specific) to aid in your COVID-19 vaccine research:

• Colorimetric protein Assays (BCA/ Bradford Assays) - Measure total protein in cell lysates with SpectraMax ABS Plus Microplate Reader
• Protein quantification (using UV absorbance) - Direct protein quantitation using the SpectraDrop Micro-Volume Microplate
• DNA/RNA quantitation (using UV absorbance) - DNA and RNA absorbance measurements using SpectraMax Microplate Readers
• Fluorescence-based DNA quantification - Quantitation of dsDNA samples using the SpectraMax Quant dsDNA Assay Kits