Measuring membrane potential
This application note provides a basic protocol for performing a membrane potential assay on the FLIPR system using the FLIPR Membrane Potential Assay Kits as well as a discussion of some of the important parameters for optimization and troubleshooting of the assay.
The kit combines the benefits of highly informative data that is comparable to patch clamping data with the benefits of high-throughput screening achievable using the FLIPR system.