Measure protein concentration (Bradford, BCA, etc)
Protein concentration can be measured directly via absorbance at 280 nm in a UV spectrophotometer, or indirectly using colorimetric methods such as BCA or Bradford assays. Protein can even be quantitated using fluorometric methods.
Here are some application notes on protein quantitation you may find of interest:
- Streamline BCA-based protein quantitation on the SpectraMax iD5 reader
- Measure total protein in cell lysates with SpectraMax ABS Plus Microplate Reader
- Protein quantitation with the EMax Plus Microplate Reader
- Direct protein quantitation using the SpectraDrop Micro-Volume Microplate
- Using the NanoOrange Protein Kit with SpectraMax Microplate Readers