Liver Spheroids (iPSC-derived hepatocytes)

Drug-induced hepatotoxicity is an important cause of liver injury and acute liver failure. Thus highly predictive assays for safety and efficacy testing are crucial for improving drug development and reducing drug attrition. Here, we discuss methods using human induced pluripotent stem cell (iPSC)-derived hepatocytes (liver spheroids), which exhibit typical characteristics and metabolism of mature cells and are ideal for use in high-content screening in early drug development.

• High-content 3D toxicity assay using iPSC-derived hepatocyte spheroids
• Multiplexed high-content hepatotoxicity assays using iPSC-derived hepatocytes
• Phenotypic Characterization of Compound Effects on iPSC-derived Cardiac and Liver Spheroids Using Fast Kinetic Fluorescence and 3D Image Analysis
• Hepatotoxicity Using iPSC-Derived Liver Cells and Beating Pattern in iPSC-Derived Cardiac Spheroids
• Determination of Hepatotoxicity in iPSC-Derived Hepatocytes by Multiplexed High Content Assays
• Phenotypic Characterization of Toxic Compound Effects on Liver Spheroids Derived from iPSC Using Confocal Imaging and Three-Dimensional Image Analysis