PLATE CELLS
Plate adherent or suspension cells into the labware of choice including culture dishes, chamber slides, and microplates. Incubate the cells at normal culture conditions (i.e. 37°C, 5% CO2). For adherent cells, incubating the plated cells overnight will allow ample time for cell attachment and growth.
96- or 384- Well Plates
Most cell cultures for high-content, high-throughput screening are run using 96- or 384-well microplates.
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TREAT WITH COMPOUNDS
If necessary for the protocol, treat the cells with compounds of interest.
Compounds can be added to cells for minutes up to several days depending on the mechanism of action of the compounds and the biological re-sponse being investigated. For example, apoptosis or receptor internalization assays generally require shorter incubation periods while multiparametric cytotoxicity assays require longer periods. Longer compound treatments may require the replacement of compounds during incubation
STAIN FOR MARKERS
Live cells can be stained with a variety of fluorophores or transfected/transduced with constructs containing fluorescent protein – peptide fusions. Manufacturer instructions should be followed when staining. Stain-ing isn’t necessary for all live cell applications and label free imaging and analysis allows for the tracking and monitoring of cells in brightfield only.
Assay Kits
Easy-to-use, robust assay kits for life science research, drug discovery and development, and bioassays. Our assay kits are optimized for use on our instruments. Screen more compounds earlier in drug discovery and enable characterization of a full concentra-tion-response profile of test compounds.
ImageXpress High-content Imaging Portfolio
Our systems for high-content imaging and analysis provide flexible scalability making it easy to evolve your system alongside your research. They feature options and modules to address your specific research including objectives, filters, imaging modes, and envi-ronmental conditions.
CONFIGURE ENVIRONMENTAL CONTROL SETTINGS
After the addition of stains and compounds, place the plate into the appropriate imaging instrument integrated with environmental control capabilities such asl gases (CO2, O2), temperature, and humidity.
For long-term time-lapse assays it is essential to maintain a natural physiological environment to ensure the health of living cells and to prevent focus drift throughout the time-lapse acquisition.
ACQUIRE LIVE CELL IMAGE
For continuous time-lapse acquisitions, configure the total length of time for the time series and the imaging intervals, i.e. image every hour for 24 hours.
Discontinuous time-lapse acquisitions can be per-formed, where the plate is taken to and from the incubator and imaged at different time points.
Automated Cell Imaging System
The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Whether running fluorescence imaging or brightfield assays, the automated imager features comprehensive preconfigured protocols for cell-based assays to shorten the learning curve, so you can start running experiments quickly.
Cellular Imaging Acquisition and Analysis Software
The CellReporterXpress Automated Image Acquisition and Analysis Software works with the ImageXpress® Pico systems. It has a clean, easy-to-learn interface for performing quantitative analysis on images acquired from automated microscopy. The software enables distributed analysis of images for increased throughput and is ideal for scaling microscopy imaging with slides or microplates.
ANALYZE LIVE CELL IMAGE
Analyze the images with cellular imaging analysis software to generate multiparametric readouts for the biological responses that are being studied. Kinetic data can be generated and reviewed for time-lapse acquisitions.