Fura-2 QBT calcium
Fura-2 dye has long been considered an important tool to measure calcium mobilization in cellular imaging, GPCR mediated intracellular calcium flux, and ion channel activation. This ratiometric dye helps correct for assay inconsistencies in dye loading or cell plating through calculating the fluorescence intensity ratio between bound and free indicators. However washing is required, increasing well-to-well variability and adding time and complexity to each assay.
The Fura-2 QBT™ Calcium Kit from Molecular Devices incorporates proven quench based technology with a ratiometric Fura-2 calcium indicator to provide a homogenous assay to minimize cell based variability, while increasing throughput through elimination of cell washing prior to detection.
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