Fluorescent Western Blot

A fluorescent western blot uses a secondary antibody conjugated to a fluorophore, so no substrate is required. A fluorescence imager is required to detect results.

Advantages:

• Greater dynamic range and better linearity than colorimetric or luminescent methods
• Can multiplex using different fluorophores

Disadvantages:

• Can be less sensitive than chemiluminescence
• Requires a dedicated fluorescence imaging system