Detection and quantitation of protein with ScanLater Western Blot Detection System
Protein detection is important for pharmaceutical and clinical research today, and western blots are among the most common methods employed for this purpose. Various techniques are used to detect proteins on western blot membranes including fluorescence and chemiluminescence. However, each technique has its limitations, and there is a continuing need to improve quantitation, accuracy, and dynamic range.
This application note demonstrates extended dynamic range due to reduced background as well as stability of detection over time and number of reads. In addition, a comparison of Scanlater Western Blot System to a chemiluminescence method demonstrates improved sensitivity.