Chemiluminescent Western Blot

A chemiluminescent western blot uses an enzyme-conjugated secondary antibody and a luminescent substrate. Results are detected using x-ray film and darkroom equipment, or a digital imaging system.

Advantages:

• Sensitivity (fg levels)
• Blots can be stripped and re-probed

Disadvantages:

• Results can be non-linear
• Signal has a short lifetime
• Equipment required for detection can be expensive and space-consuming