Cell cycle analysis
Transition of a cell through the cell cycle can be monitored using fluorescently-labeled cell cycle proteins that are cyclically expressed and degraded. FUCCI technology is based on the overexpression the cell cycle-dependent proteins geminin and Cdt1, respectively fused to a green fluorophore and a red fluorophore. Cdt1 levels peak in G1 phase, so cells in G1 appear green; geminin levels rise in late S, G2 and M phase, so cells in these phases appear red.