CatchPoint cGMP Assay Principle

The cGMP in the sample or standard competes with horseradish peroxidase (HRP)-labeled cGMP conjugate for binding sites on the anti-cGMP antibodies. In the absence of cGMP, most of the HRP-cGMP conjugate is bound to the antibody. Increasing concentrations of cGMP competitively decrease the amount of bound conjugate, thus decreasing measured HRP activity