cAMP Assays (GPCR, Gi/Gs coupled)

Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of Gi/Gs protein-coupled receptors. cAMP levels can be monitored using fluorescent molecules that bind to cAMP and are detected using a fluorescence plate reader. Some cAMP assays require other detection modes, such as time-resolved fluorescence.

Here are a few application notes you may find of interest:

• Complete cAMP workflow solution using the CatchPoint cAMP Fluorescent Assay Kit
• Detect GPCR activity with the cAMP-Gs HiRange HTRF assay
• HTRF cAMP HiRange Assay on SpectraMax Multi-Mode Microplate Readers
• HTRF cAMP dynamic 2 and IP-One assays on the SpectraMax M5e reader