Assembly

Target gene (gene of interest) is ligated into a vector with desirable characteristics, forming a recombinant plasmid. This can be done via multiple techniques, including Gibson Assembly or Golden Gate Assembly.

01

Transform

The ligation products are introduced into competent bacterial cells, allowing microbial uptake of the engineered plasmid and production of multiple plasmid copies.

02

Colony Plating & Screening

Microbial cells are spread onto solid agar plates to grow into individual colonies, which are then screened to identify those with the desirable characteristics for picking.

03

QPix Microbial Colony Picker

Colony Picking

Once desirable colonies have been identified, they can be picked from solid agar plates and transferred to liquid media for overnight growth and incubation

04

Overnight Incubation

Growth plates with liquid media are sealed and transferred to shaking incubators to allow the microbes to divide, producing more copies of the recombinant plasmid.

05

Mini Prep

Microbes are lysed to extract the recombinant plasmids. Plasmids are then purified using column or bead-based methods to obtain highly pure DNA

06

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