Assay Kit Mechanism

Fura-2 QBT Calcium Kit utilizes Fura-2, AM a calcium sensitive dye that is absorbed into the cell's cytoplasm during incubation, where lipophilic blocking groups are cleaved resulting in a negatively charged fluorescent dye that stays within the cell. Upon target activation, intracellular calcium is released, binding with the dye, thereby increasing fluorescence intensity. This kit utilizes an extracellular masking technology to block background fluorescence and increase your assay's signal window, and decrease assay steps.