Analyze cell area using transmitted light or fluorescence image
In this assay, cell proliferation, migration, and spreading all contributed to the cells’ ability to close the wound. Automated timelapse imaging using transmitted light or live cell-compatible fluorescence provided the means to measure the progress of the “healing”. The images from every time point may be objectively analyzed with high-content image analysis software so that the data can be plotted as a timecourse or evaluated individually at specific time points.