3D cardiac spheroids
There is an increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling tissue biology and toxicity assessment. The development of quantitative assays in higher throughput using 3D cultures is an active area of investigation. Studying the formation of 3D spheroids derived from human induced pluripotent stem cells (iPSC), using both high-content imaging (HCI) and fast kinetic fluorescence imaging helps measures the impact of various compounds on the beating rate and pattern of cardiac spheroids as monitored by changes in intracellular calcium levels with calcium-sensitive dyes.