ON-DEMAND WEBINAR
Leverage automated, end-to-end workflows to enable complex organoid assays
Improving lab scalability for advanced drug discovery
3D organoids are becoming popular in drug discovery and disease modeling as they better represent biologically relevant microenvironments. However, the protocols for organoid assays are complex and often require labor-intensive monitoring by highly trained specialists. In addition, there is a lack of clarity about automation’s contribution to increasing assay throughput, scalability, and workflow reliability, while still reducing overall cost.
In this webinar, experts share strategies to deliver relevant complex biological models at scale and clarify the role automation plays in the future of drug discovery. In addition, they examine how to address the complexities that come with translating bench protocols to automated methods and discuss best practices for automating cell and organoid workflows. Key takeaways include:
- Tools to increase organoid assay throughput and automation
- Analysis approaches to gain more insight into complex systems, disease phenotypes, and compound effects
- Methods to automate cell culture and cell analysis of 3D organoids
- Examples of automated seeding, media exchange, organoid development monitoring, and phenotypic change evaluation
https://share.vidyard.com/watch/gQNcAxBxoh7qKKKtWyP8qz
Q&A
How many wells can be plated at a time with Matrigel domes without the Matrigel solidifing in the tip? Is the matrigel kept on 4C?
We can print at least 2-3 full 24 well plate and we use pre-chilled Matrigel mix and cooling alp.
We have recently acquired the confocal module for our IXM and I'm wondering if you have material that you can share on how to do the various analysis you presented here today?
We have numerous training materials and app notes available on our website, also ready-to go protocols. Please contact us for more info - Contact Us | Molecular Devices.
How do you handle 24 well plates with a 96 pipettor? Do you need to use the 8 pipettor?
We have the ability to select tips, this allows us to load 24 tips onto the head. We can also reformat these tips. For example, if you have a 96 tip box on the deck, you can place potentially 4 empty boxes and rearray the tips out ahead of time, this can be done live or during another process, such as an on-deck incubation, or it could be pre-synged, so you have the ability to take a section of tips and reformat them to any pattern and then reload the entire set onto the head to do a 24 well plate.
For the mid-brain organoid work, do you know if that was performed on a single liquid handler or a factory?
This is typically performed on a single system with an incubator and liquid handler.
Typically, we place a drop of Matrigel in a well and then spread it in each well with zigzag motion of the pipet tip. How is this done in your system?
We put a droplet in and it will attract the pipet tip, it will spread slightly, but it stays as a dome and you can regulate the size by volume. For example, for a 96 well plate we would use 25-30 microliters.
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