ON-DEMAND WEBINAR
Automate your 3D biology high-throughput workflows
https://share.vidyard.com/watch/4doQ6U8MMkt9SGoXKP5D84
Q&A
Can the microscope focus on individual spheroids located in a single droplet at different focal lens positions automatically?
Yes, it can. We provide a customizable laser autofocus setup which is a great fit for applications like these. Laser autofocus can be programmed to focus on any user-defined focal planes within the working distance in an automated fashion.
Does the imaging system require pre-labeled cells or can it be used for spheroid staining with antibodies and fluorophores?
It can be used for both the immunostained samples and live samples expressing fluorescent protein like GFP, RFP, mCherry. Our system can be equipped with 8 color lasers that enables imaging a wide range of commercially available fluorophores along with a transmitted-light channel for brightfield imaging.
Organoid technology is patented technology - so is the model you’re using ideally organoid or spheroid only?
Our technology is organoid, spheroid, and tissue model agnostic. In this case, these were primarily spheroids that we developed and used - although we have partnerships with others that have organoids that they’ve developed internally and we’re working with them to automate the process.
How much translational capability of your model could be used in drug discovery
Automation can be used in many ways for drug discovery, including evaluation of tumor spheroid work. One of the major objectives for implementing automation is to make that process more turnkey and increase throughput.