Molecular Devices at SLAS 2023: All our resources at your fingertips!
The Society for Lab Automation and Screening (SLAS) 2023 conference was another exciting year to learn about innovative laboratory technologies. Whether you attended in-person or visited us online at our virtual events page, we were excited to share new methods and protocols to automate your complex biology workflows.
In case you missed any of our presentations or would just like to take a deeper look into the research, we’ve compiled all our resources below for you to explore at your leisure.
Tutorials
TUTORIAL: Automating Organoid Models and Assay Workflows
Presented by Oksana Sirenko, PhD and Jay Hoying, PhD, Advanced Solutions
Tissue organoids and other 3D biological models are advancing biomedical research – including drug and target discovery – by capturing more of the relevant tissue biology. To get the most out of this research, scientists are creating complex, in vitro models that need to be deployed quickly using turnkey workflow solutions that align the automation to the biology. In this tutorial, we will describe an automated workflow to form, bioprint, culture, transfer, assay, and analyze tissue organoid models using the BioAssemblyBot® 400 bioprinter and ImageXpress® Confocal HT.ai High-Content Imaging System. In addition, we will explore different tissue models, including patient-derived cancer organoids (PDOs) from Cellesce, and discuss their use with various automated workflows. This session is sure to provide an engaging and in-depth discussion of advanced tissue modeling and assaying.
TUTORIAL: FUJIFILM Cellular Dynamics - Using Differentiated iPSCs to Build Ready-to-use 3D Models
Presented by Oksana Sirenko, PhD and Coby Carlson , PhD, FUJIFILM
Human iPSC cells are critical for disease modeling, toxicity assessment, and for providing highly relevant human model systems for basic discovery research. We, along with our industry partner Molecular Devices, will highlight a series of new data for co-culture and 3D systems that demonstrate our ability to use these differentiated cells to enhance screening models. This presentation will include data for our novel iCell® NeuroSpheres and iCell CardioSpheres.
Posters
POSTER: Automating patient-derived colorectal cancer organoids for high-throughput screening
Presented by Angeline Lim, PhD
For this poster, we developed an end-to-end, automated workflow starting with assay-ready colorectal cancer organoids that tracked the effects of various compounds on colorectal organoid size, morphology, texture, and additional morphological and phenotypic readouts. Overall, our results show the superior potential of PDOs vs. other tissues in both precision medicine and high-throughput drug discovery applications when using automation with high-content imaging.
POSTER: Automation of 3D bioprinting assays for high-content imaging and assessment of compound effects
Presented by Prathyushakrishna Macha, PhD
The automation of a 3D cell model results in a significant reduction in the time and effort involved, as well as an increase in assay precision and throughput. Here we describe methods for an automated generation of organoids and 3D models using automated 3D bioprinting. An increase in throughput and ease of operation was achieved through automation. Also, imaging and data analysis methods provided valuable information about complex compound effects in 3D printed and cell-tissue-engineered models
POSTER: Leveraging Automated Workflows to Enable Complex Organoid Assays
Presented by Oksana Sirenko, PhD
In this poster we demonstrate the tools for increasing throughput and automation in organoid assays and provide strategies aimed at enabling reliable, scalable, and reproducible cell culturing, monitoring, imaging, and analysis of complex biological models.
POSTER: Novel analysis of neural outgrowth in 3D human brain micro-tissues
Presented by Angeline Lim, PhD
In this study, we reconstructed RealBrain® micro-tissues in 3D and developed a method to quantify the number of neurons and their respective outgrowths. With this approach, we confirmed the efficacy of the compounds with the quantification of neurite outgrowth analysis and found that NGF and PD acid significantly increase, while Rotenone reduces the number of neurites compared to the control. Thus, this type of analysis can be used in multiple applications, such as assessing the effects of compounds on neurons and neural networks.
POSTER: Optimizing Animal free CloneDetect assay with Protein G or A to maximize the sensitivity of real-time detection of human IgG antibody production for therapeutic protein engineering and cell line development using ClonePix System
Presented by Paula Zadek, PhD
In this study, we explore the usage of recombinant Pro A or Pro G combined with CloneDetect K8495 to enhance the fluorescent signal for IgG detection/quantification. The results have shown that adding Pro G at the final concentration of 1.0 ug/ml produced the best outcome compared to the control assay without adding Pro G optimizer. The combination of Pro G and CloneDetect K8495 amplified the fluorescent signal and improved detection, due to the stabilization of the complexes formed by the target protein, Pro G, and CloneDetect reagent. This approach can be applied to a variety of cell line models that express proteins that bind to the stabilized complex formed with CloneDetect.
POSTER: Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager
Presented by Prathyushakrishna Macha, PhD
In this study, we demonstrate a workflow to increase throughput and automate single-cell isolation procedures and clonal outgrowth compared to limiting dilution method. Combining single-cell dispensing and imaging into a workflow provides an innovative and simple impedance-based method for the isolation of intact single cells and imaging. Its incorporation into an automated platform allows the increase in dispensing throughput and reliability and has the potential to facilitate gene editing. In addition, it can generate multiple engineered cell lines that carry specific indel and can facilitate the development of several bioprocess developments or drug screening studies.
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