SCIENTIFIC POSTER
Leverage automated workflows to enable complex organoid assays
3D organoids are increasingly popular in drug discovery and disease modeling, however, their complexity can be a hurdle for their wider adoption in research and drug screening.
Here, we describe how to alleviate the hurdles of the labor-intensive manual protocols by using automation. Accordingly, we developed automation protocols for the entire 3D culture workflow and increase the throughput and accuracy of organoid assays.
The automated methods utilize an integrated workcell, providing automated cell plating, culture monitoring, media exchange, and a high-content imaging. In addition, the method allows automation of compound addition, cell staining, and end point assays.
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Imaging
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ImageXpress Confocal HT.ai; MetaXpress High-Content Image Acquisition and Analysis Software; IN Carta Image Analysis Software
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7014u000001AXaRAAW
Title
Leverage automated workflows to enable complex organoid assays
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