SCIENTIFIC POSTER
Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager
Single-cell dispensing and screening of cell lines for monoclonality verification using the impedance-based single-cell dispenser and high-throughput fluorescence-based imager
There is an unmet need in the industry for a device that allows the fast and efficient isolation of single cells while preserving their integrity and providing insurance for their clonality. Performing gene editing, single-cell dispensing, and screening to develop a stable monoclonal cell line is a long and labor-intensive process. The optimization of these steps through different approaches, such as high-throughput screening and/or automation, can increase the efficiency and yield of the monoclonal cell-line development process. In this poster, we show the impedance-based single-cell dispenser and automated fluorescence-based high-throughput imaging screening to obtain monoclonal cell lines through robust single-cell selection.
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Advance your microbial and mammalian clone screening with proven, automated technologies
Molecular cloning is a set of methods, which are used to insert recombinant DNA into a vector—a carrier of DNA molecules that will replicate recombinant DNA fragments in host organisms. This is a very laborious process when done manually requiring hundreds of plates and kits. There are several areas where errors or contamination can occur. Automating the process can dramatically reduce labor while increasing throughput and efficiency twofold.
Introducing the DispenCell Single-Cell Dispenser
The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy, and gentle single-cell isolation. DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop.
- Immediate and traceable proof of clonality: The instrument is fitted with a sensing tip that detects the passage of cells, allowing users to check for proof of clonality immediately after the cells are dispensed. All dispensing data is automatically stored, allowing for easy generation of a clonality report.
- As gentle as manual pipetting: DispenCell has unique technology which allows for extremely gentle handling of the cell sample. Cells are exposed to no more pressure than with manual pipetting (less than 0.1 psi).
- Compact: The small footprint of the DispenCell allows it to fit perfectly into any laboratory setting.
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