3D Cell Culture Insights

FREE EBOOK:

Capture True 3D Cell Culture Insights

Gain new insights using advanced high-throughput and high-content imaging systems

Capture True 3D Cell Culture Insights

What you will find in this eBook

Development of more complex, biologically relevant, and predictive cell-based assays for compound screening is a primary challenge in drug discovery. The integration of three-dimensional (3D) assay models is becoming more widespread to drive translational biology. Specifically, 3D cultures offer the advantage of closely recapitulating aspects of human tissues including the architecture, cell organization, cell-cell and cell-matrix interactions, and more physiologically-relevant diffusion characteristics.

This ebook highlights the 3D cell culture imaging and analysis advantage and includes a few 3D cell model applications as well as tips and techniques for optimizing your 3D assay workflow.

Applications

  • Physiologically-relevant tissue models using high-throughput organ-on-a-chip platform
  • Functional evaluation of neurotoxic and neuroactive compound effects in iPSC-derived 3D neural co-cultures
  • High-content assay for morphological characterization of neuronal development in 3D matrix
  • Assessment of drug effects on cardiomyocyte physiology using human iPSC-derived cardiac spheroids
  • Using spheroid models to develop relevant highthroughput assays with quantitative endpoints

Tips and Techniques

  • Automated 3D cell culture and screening by imaging and flow cytometry
  • Automation of 3D spheroid cultures in ultra-low attachment plates
  • Multi-parameter imaging assay for measuring toxicity in a tumor model
  • Measuring mitochondrial shape and distribution
  • Increase sensitivity in no-wash assays using confocal imaging

Sample pages

3D Imaging Advantage

3D Imaging Advantage

Organ-on-a-chip Platform

Organ-on-a-chip Platform

Neural Co-culture Platform

Neural Co-culture Platform

3D Hydrogel

3D Hydrogel

Automated 3D Cell Culture and Screening

Automated 3D Cell Culture and Screening

SIMPLIFY 3D CELL CULTURE IMAGING AND ANALYSIS

Register to download your
free eBook today

Whatever your imaging requirement, we have a solution
that will meet the needs of your lab and your budget.

3D Cellular Imaging Systems

Our ImageXpress portfolio of cellular imaging systems and analysis software offers innovative capabilities for evaluating complex and emerging 3D cell culture-based models such as:

  • Organoids
  • Spheroids
  • Whole organisms
  • Induced Pluripotent Stem Cell (iPSC)-derived cell models
  • Organ-on-a-chip technologies
SelectScience

Spheroids

ImageXpress Pico

Automated Cell Imaging System

ImageXpress Pico

Spheroids

ImageXpress Nano

Automated Imaging System

ImageXpress Nano

Spheroids

ImageXpress Micro 4

High-Content Imaging System

ImageXpress Micro 4

Spheroids

ImageXpress Micro Confocal

High-Content Imaging System

ImageXpress Micro Confocal

CAMERA
5 megaPixel CMOS
4.7 megaPixel CMOS
>4 megaPixel CMOS
>4 megaPixel CMOS
FIELD OF VIEW 10X
1.93 mm2
1.85 mm2
1.96 mm2
1.96 mm2
OBJECTIVES
4x-63x Air
2x-60x Air
Air (1x-100x), Oil (40x, 60x, 100x)

Air (1x-100x),

Oil (40x, 60x, 100x),

and Water (20x, 40x, 60x)

FILTERS CHANGER
5 position filter cube changer
5 position filter cube changer
5 position filter cube changer

8 position emission wheel and

5 position dichroic filter wheel

AUTOFOCUS
LED+ image autofocus
Laser+image autofocus
Laser+image autofocus
Laser+image autofocus
PLATES
Up to 384-well plates
Up to 1536-well plates
Up to 1536-well plates
Up to 1536-well plates
TEMPERATURE CONTROL
OPTION
done
done
done
done

If you requested more information, one of our team will contact you shortly.