Harvard Medical School uses the QPix colony pickers to map networks of macromolecular interactions to help understand phenotype to gene relationship

The Challenge

At the Dana-Farber Cancer Institute, the Center for Cancer Systems Biology (CCSB) utilizes experimental and computational strategies to uncover complex biological interactions. Using a wealth of technology platforms and bioinformatics tools, they have generated protein-protein interaction (PPI), or “interactome”, maps for a variety of organisms. High-quality binary PPIs are generated using variants of yeast two-hybrid methodologies combined with validation using orthogonal in vivo and in vitro approaches. Unbiased experimental data can be integrated with literature research and computational analysis to aid their discoveries.

The Solution

The QPix™ 460 provides this experimental ability by screening and picking microbial libraries in a high-throughput manner. The QPix helps them increase both the speed as well as volume at which protein-protein interactions which are discovered, enabling them to rapidly expand the high-quality interactome maps.

QPix Microbial Colony Pickers

Details

Products Used

The QPix™ 400 series of microbial colony pickers offer you the unique option to simultaneously detect colonies and quantify fluorescent markers in a pre-screening step before picking. Our QPix systems are used worldwide in over 600 installations in research institutes, sequencing facilities, biotech and pharmaceutical companies. QPix robotics developed a famous reputation for reliability and accuracy in sequencing centers during the Human Genome project.

More than just colony pickers, these systems enable scientists to:

• Automate workflows from sampling and spreading to picking.
• Pick the right colonies every time
• Efficiently manage large, diverse populations for: protein expression, biofuel research, enzyme evolution, phage display, DNA sequencing, library generation and management.

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