APPLICATION NOTE
Quantify TNFα secretion by THP-1-derived macrophages with an AlphaLISA assay
A major cellular component of the tumor microenvironment, tumor-associated macrophages are often associated with poor prognosis and are under investigation as potential targets for anticancer therapy.
Macrophages are a type of white blood cell that plays a role in detecting and destroying infectious microorganisms, removing dead cells and debris, and in wound healing.
In this application note, effects of several anti-inflammatory compounds on TNFα secretion by THP-1-derived macrophages were measured using the AlphaLISA High Performance (HP) Human Tumor Necrosis Factor alpha (TNFα) Detection Kit. Using the SpectraMax i3x Multi-Mode Microplate Reader and this homogeneous assay, picogram levels of TNFα were detected in culture medium removed from THP-1 cells activated with PMA and LPS, and further treated with the compounds SB202190, stattic, and resveratrol.
Learn more about the systems behind the methods:
SpectraMax i3x Multi-Mode Microplate Reader
Western blot, imaging, and injectors on one configurable microplate reader
The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user-configurable detection modules, the reader provides you with the ability to explore cellular pathways and protein expression on one microplate reader.
SoftMax Pro Software
The most published microplate reader control and data analysis software
SoftMax® Pro Software for Windows 10 is designed to provide the simplicity, flexibility and power required for advanced data analysis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from another source to simplify analysis and reporting. Compliance tools are available for regulated laboratories providing end-to-end chain of custody.
SpectraMax MiniMax 300 Imaging Cytometer
Observe the viability of your cells with the field-upgradeable cellular imaging option for the SpectraMax i3/i3x Multi-mode Detection Platform
The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability.
- Simplified workflow: Add cellular imaging assays in a small footprint
- No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using StainFree™ Technology
- More data: Multi-parametric cell-based assays with brightfield and green and red fluorescent imaging
- Familiar software: Set up, image, and analyze cells in a snap using SoftMax® Pro Microplate Data Acquisition & Analysis Software
- Higher sensitivity: Easily measure low cell concentration samples
- Lower variance: Normalize your plate reader assay data to the number of cells in each well
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