APPLICATION NOTE
Quantify TNFα secretion by THP-1-derived macrophages with an AlphaLISA assay
A major cellular component of the tumor microenvironment, tumor-associated macrophages are often associated with poor prognosis and are under investigation as potential targets for anticancer therapy.
Macrophages are a type of white blood cell that plays a role in detecting and destroying infectious microorganisms, removing dead cells and debris, and in wound healing.
In this application note, effects of several anti-inflammatory compounds on TNFα secretion by THP-1-derived macrophages were measured using the AlphaLISA High Performance (HP) Human Tumor Necrosis Factor alpha (TNFα) Detection Kit. Using the SpectraMax i3x Multi-Mode Microplate Reader and this homogeneous assay, picogram levels of TNFα were detected in culture medium removed from THP-1 cells activated with PMA and LPS, and further treated with the compounds SB202190, stattic, and resveratrol.
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Product family
SMax iX
Product primary application
SpectraMax i3x Multi-Mode Microplate Reader; SoftMax Pro Software; SoftMax Pro GxP Software; SpectraMax MiniMax 300 Imaging Cytometer
CMP
7014u000001AX20AAG
Title
Quantify TNFα secretion by THP-1-derived macrophages with an AlphaLISA assay
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