APPLICATION NOTE
Optimizing performance of Transcreener fluorescence polarization assays with the SpectraMax i3x Multi-Mode Microplate Reader
The Transcreener FP Assays are a single step, competitive immunoassay for direct detection of nucleotides with a far-red fluorescence polarization (FP) readout.
The use of a far-red tracer minimizes interference from fluorescent compounds and light scattering. The Transcreener FP Assays are designed specifically for high-throughput screening (HTS) with a single addition, mix-and-read format.
In this application note, we describe the optimal instrument parameters used to validate the SpectraMax® i3x Multi-Mode Microplate Reader with the Transcreener® ADP2 FP assay from BellBrook Labs.
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Product family
Microplate Reader
Product primary application
ImageXpress Micro Confocal High-Content Imaging System; MetaXpress High-Content Image Acquisition and Analysis Software; SpectraMax i3x Multi-Mode Microplate Reade
CMP
7014u0000017gD7AAI
Title
Optimizing performance of Transcreener fluorescence polarization assays with the SpectraMax i3x Multi-Mode Microplate Reader
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