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APPLICATION NOTE
Enhancing cell line development for cultured meat production: A gentle and efficient single clone generation pipeline
- Our gentle and efficient single clone generation pipeline ensures higher clonality rates, enabling more precise and consistent cell line development for cultured meat production.
- By utilizing the DispenCell system, improve the efficiency of cell line generation when compared to traditional methods, saving time and resources.
- Minimize cell stress and optimize culture conditions to ensure robust and sustainable cell lines.
![Enhancing cell line development for cultured meat production](./media_1cd510c18774cd7034f81382247c3ab1767826482.png?width=750&format=png&optimize=medium)
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Cultured meat (CM) is an emerging technology, which is crucial to supply the increasing meat demand while being more environmentally and animal friendly than conventional meat production.
Current workflows to generate single cell clones employ limiting dilution, which inherently is rather inefficient, or flow cytometry-based cell sorting, which is stressful especially for primary cells. As such we aimed to establish a gentle and efficient single clone generation pipeline. Here, we compared the clone generation with the DispenCell in terms of clonality, efficiency and survival as well as proliferation with a flow-based system.