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SpectraMax Paradigm Multi-Mode Microplate Reader

A compact, capable, and user-upgradeable microplate reader that enables you to do more, with less.

Future-proof your lab with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Get the system that delivers the data you need today, and when you need more, transform your reader with a detection-mode addition upgrade that takes less than two minutes. The SpectraMax Paradigm reader can do absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence measurements. Detection cartridge modularity allows you to configure the system to meet your current needs, while providing flexibility to address future applications.

Benefits

  • Flexible design allows for single or dual excitation and emission, to address existing needs and future expansion
  • User upgradable microplate reader in < 2 minutes
  • High speed detection
  • Excellent sensitivity and performance
  • Applications at your fingertips

Features

  • Array of detection cartridge capabilities
  • Automatic Z-height optimization (both top and bottom read configurations)
  • Fluorescence measurements no longer require you to adjust the gain
  • Patented LED intensity adjustment
  • Up to 1536-well format
  • Temperature control
  • Monochromator-based absorbance
  • Filter-based fluorescence
  • TUNE Cartridge available for unmatched sensitivity in wavelength tunable detection
  • Custom Cartridge development available to meet your specific research needs
  • SpectraDrop™ Plate protocols enabling micro volume applications measurement are included
  • Seamlessly integrate with StakMax® Microplate Handling Stacker through SoftMax® Pro Software interface

Assay Kits

  • We offer optimized assay kits for use with the SpectraMax Paradigm reader.

SpectraMax® Paradigm® features future-ready applications that can be installed and ready to use in less than two minutes. With over 20 user-installable detection cartridges available, including custom cartridges, users can easily expand their microplate reader to meet their current and future research needs.

  • Fluorescence Intensity
    • DNA/RNA/protein quantitation
    • Endpoint qPCR
    • Green fluorescent protein
    • Receptor-ligand binding
  • Fluorescence Polarization
    • SNP genotyping
    • Protein-protein interactions
    • Drug receptor studies
  • Absorbance
    • DNA/RNA and protein purity
    • ELISA
    • Cell viability
    • Enzyme kinetics
    • Low volume applications
  • Time-Resolved Fluorescence
    • Enzyme Activity
    • TR-FRET Eu+3 chelates
    • Protein-protein interactions
    • Western blot detection
  • Glow Luminescence
    • LIA
    • Reporter gene
    • cAMP
    • Toxicity
    • Screens
    • Kinetics

SoftMax® Pro Microplate Data Acquistion & Analysis Software, Molecular Devices industry leading all-in-one data acquisition and analysis software comes standard with the Paradigm Platform. SpectraDrop™ Plate protocols that enable Micro Volume applications measurement are also included.

Benefits

  • The most extensive library of "Ready-to-Run" application protocols to get you started faster
  • Advanced calculations including non-linear fitting metrics for in-depth analysis
  • Syntax Helper to simplify complex calculations
  • Tools to customize reporting and exporting of data
  • GxP support for 21 CFR Part 11 compliance is available

 

softmaxpro-sw-v5_450_0.jpg

 

 

 

 

General Specifications

Light source
High Powered LED or Flash Lamp
Plate formats
6- to 1536-well
Temperature range
4°C above ambient to 45°C
Uniformity
± 0.75°C
Shaking
Linear, Orbital
Endpoint measurement
All Modes
Kinetic measurement
All Modes
Well area scanning
All Modes
Wavelength scanning
Photometry
Dimensions W Ã-H Ã-D cm (in.)
39 (15.4) -45 (17.7) -63 (24.8)
Weight, without cartridges
45 kg (99 lbs)
Power source
100-240 VAC, 2 A, 50/60 Hz
Robotic compatible
Yes
Linear dynamic range
> 5 decades

Technical Specifications

Fluorometry (FI) (Fluorescein)
Guaranteed*
Optimized
96-well (200 μL)
0.100 fmol
0.020 fmol
384-well (75 μL)
0.100 fmol
0.012 fmol
1536-well (8 μL)
0.030 fmol
0.003 fmol
Fluorescence Polarization (FP) (Fluorescein 1 nM, SD)
96-well (200 μL)
3.0 mP
0.5 mP
384-well (75 μL)
3.0 mP
0.5 mP
1536-well (8 μL)
6.0 mP
1.5 mP
Time Resolved Fluorescence (TRF) (Europium)
96-well (200 μL)
10.0 amol
2.5 amol
384-well (75 μL)
5.0 amol
1.0 amol (100 μL)
1536-well (8 μL)
1.5 amol
0.6 amol
Luminescence (Lum) (ATP-Glow)
96-well (200 μL)
0.50 fmol
0.30 fmol
384-well (50 μL)
0.50 fmol
0.30 fmol
1536-well (8 μL)
0.20 fmol
0.15 fmol
 * For properly functioning, operated and maintained equipment.
 
Photometric
Wavelength Range
230-1000 nm
Wavelength Selection
Monochromator (1.0 nm increments)
Wavelength Bandwidth
4.0 nm
Wavelength Accuracy
± 1 nm
Wavelength Repeatability
± 0.5 nm
Photometric Range
3.5 OD
Photometric Resolution
0.0001 OD
Photometric Accuracy
± (2% + 0.010 OD), 0-2 OD @ 405 nm
Photometric Precision
± (1% + 0.005 OD), 0-2 OD @ 405 nm

Related Videos

This video features the SpectraMax Paradigm Multi-Mode Microplate Reader with the TUNE Cartridge.

 

 

 

 

 

Related Collateral

eBook

Brochures/Datasheets

Applications

Posters

Related Webinars

 

 

SpectraMax® Paradigm Multi-Mode Microplate Reader

Product

Part Number

SpectraMax Paradigm Multi-Mode Microplate Reader with SoftMax Pro Software PARADIGM
SpectraMax Absorbance (ABS) Detection Cartridge
0200-7000
SpectraMax Tunable Wavelength (TUNE) Detection Cartridge
0200-7050
SpectraMax Multi-mode (MULTI) Detection Cartridge
0200-7001
SpectraMax Fluorescence Intensity (FI) (coum-fluor) Detection Cartridge
0200-7002
SpectraMax Fluorescence Intensity (FI) (fluor-rhod) Detection Cartridge
0200-7003
SpectraMax Fluorescence Intensity (FI) (Cy3-Cy5) Detection Cartridge
0200-7004
SpectraMax Fluorescence Intensity (FI) (CFP-YFP) Detection Cartridge
0200-7005
SpectraMax Fluorescence Intensity (FI) GeneBLAzer Detection Cartridge
0200-7006
SpectraMax Fluorescence Intensity Dual Label (FI-DL) (MultiTox-Fluor) Detection Cartridge
0200-7007
SpectraMax Time Resolved Fluorescence (TRF) Detection Cartridge
0200-7008
SpectraMax Fluorescence Polarization (FP) (fluorescein) Detection Cartridge
0200-7009
SpectraMax Fluorescence Polarization (FP) (rhodamine) Detection Cartridge
0200-7010
SpectraMax Cisbio HTRF Detection Cartridge
0200-7011
SpectraMax Glow Luminescence (LUM) Detection Cartridge
0200-7012
SpectraMax Dual Color Luminescence (LUM) (Chroma-Glo) Detection Cartridge
0200-7013
SpectraMax  Glow Luminescence (LUM) (96) Detection Cartridge
0200-7014
SpectraMax Glow Luminescence (LUM) (384) Detection Cartridge
0200-7015
SpectraMax Dual Color Luminescence (LUM) (BRET2TM) Detection Cartridge
0200-7016
SpectraMax AlphaScreen 384 STD Detection Cartridge
0200-7017
SpectraMax AlphaScreen 384 HTS Detection Cartridge
0200-7018
SpectraMax AlphaScreen 1536 HTS Detection Cartridge
0200-7019
 

Other Accessories

 Product Name
Part Number
SpectraDrop™ Micro-Volume Microplate Starter Kit
0200-6262
SpectraDrop™ Micro-Volume Microplate HTS Kit
0200-6267
Multi-Mode Validation Plate for SpectraMax® Paradigm® & FilterMax™ F3/F5 Systems
0200-7200

Number of Citations*: 3,750

Latest Citations:
For a complete list, please click here.

Rootstock-induced physiological and biochemical mechanisms of drought tolerance in sweet orange

LP Gonçalves, TFO Alves, CPS Martins… - Acta Physiologiae …, 2016 - Springer
... One unit of SOD activity was defined as the amount of enzyme required to cause
50 % inhibition of reduction of NBT measured at 560 nm on a spectrophotometer
(SPECTRAMax Paradigm, Molecular Devices, Sunnyvale, USA). ...

Involvement of Bcl-2 Signal Pathway in the Protective Effects of Apigenin on Anoxia/Reoxygenation-induced Myocardium Injury

C Chen, H He, Y Luo, M Zhou, D Yin… - Journal of cardiovascular …, 2016 - journals.lww.com
Abstract: Apigenin is a type of flavonoids, which has been
demonstrated to protect myocardium agains.

Melanin pigmentation gives rise to black spots on the wings of the silkworm Bombyx mori

K Ito, M Yoshikawa, T Fujii, H Tabunoki… - Journal of Insect …, 2016 - Elsevier
... 2011). Because the absorption maximum of melanin is at 405 nm, the amount of
melanin was determined spectrophotometrically using a SpectraMax Paradigm reader
(Molecular Devices, Sunnyvale, CA) (Akihisa et al., 2010). ...

Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes

KA Tomcik, WJ Smiles, DM Camera, HM Hügel… - European journal of …, 2016 - Springer
... Cell viability was assessed by measuring the fluorescence excited at 550 nm and read at 600
nm using a SpectraMax Paradigm plate reader (Molecular Devices, Sunnyvale, CA). All assays
were performed in quadrupli- cate and independently repeated. Page 3. Eur J Nutr 1 3 ...
 

Rose bengal uptake by E. faecalis and F. nucleatum and light-mediated antibacterial activity measured by flow cytometry

D Manoil, A Filieri, J Schrenzel, S Bouillaguet - Journal of Photochemistry …, 2016 - Elsevier
... dimethylsulfoxide (DMSO – Sigma-Aldrich, Buchs, CH). The absorbance of both solutions
in the range of 350–650 nm was measured using a Spectramax Paradigm
spectrophotometer (Molecular Devices, CA, USA) (Fig. 1A). A dental quartz ...
 

[HTML] A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Z ZhangB WangQ Hu, M Sommerfeld… - Biotechnology and …, 2016 - Wiley Online Library
... In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of
heterotrophically grown cells to specific light/nutrient ... Cell growth was monitored by measuring
optical density at 750 nm with a UV-vis spectrophotometer (SPECTRAmax 340PC, Sunnyvale, ...

Optimizing surface-engineered ultra-small gold nanoparticles for highly efficient miRNA delivery to enhance osteogenic differentiation of bone mesenchymal stromal …

M Yu, B LeiC GaoJ YanPX Ma - Nano Research, 2016 - Springer
... China). After further incubation for 5 min, the OD was measured at 610 nm by a
Spectramax paradigm (Molecular Devices). The ... USA). Luminescence measurements
were performed on a Spectramax paradigm (Molecular Devices). At ...

Functional characteristics of the natural polymorphisms of HIV-1 gp41 in HIV-1 isolates from enfuvirtide-naïve Korean patients

YH Shin, CH Yoon, HJ Yang, H Lim, BS Choi, SS Kim… - Archives of …, 2016 - Springer
... to infect cells in triplicate. At 48 h postinfection, the infectivity was determined by
measuring luminescence using a SpectraMax Paradigm Multi-Mode Microplate
Reader (Molecular Devices). IC50 determination for enfuvirtide ...

Protein coingestion with alcohol following strenuous exercise attenuates alcohol-induced intramyocellular apoptosis and inhibition of autophagy

WJ Smiles, EB Parr, VG Coffey… - American Journal of …, 2016 - Am Physiological Soc
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* as of December 6, 2016.  Source: Google Scholar.