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ScanLater Western Blot Detection Cartridge

Transform your SpectraMax i3x or SpectraMax Paradigm System into a western blot reader

There are often a great many western blots along the path between imagination and understanding. Now you can read them using the advanced optical technology that's already in your lab — inside your microplate reader. The ScanLater™ Western Blot Detection System includes the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Assay Kit, and image acquisition powered by SoftMax® Pro Software. In just minutes, you can install the ScanLater Western Blot Detection Cartridge in a SpectraMax® i3x or SpectraMax® Paradigm® System to add western blot detection capability, rather than buying a dedicated western blot detection system. See more, and do more, with your microplate reader.

The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3x and SpectraMax Paradigm Multi-Mode Platforms. Leveraging this novel technology, researchers can:

  • Add western blot detection to their microplate reader within 2 minutes
  • Eliminate time-dependent substrate addition steps
  • Maintain femtogram to picogram protein sensitivity comparable to traditional western blot methods
  • Sustain blot signal stability for up to 30 days
  • Use a single software platform SoftMax Pro Software, to run both microplate and western blot detection assays

Features:

  • User-installable cartridge for the SpectraMax i3 and Paradigm Multi-Mode Platforms
  • Time-resolved fluorescence detection
  • Xenon flash lamp excitation source
    • 340/80 nm excitation range
    • 616/10 nm emission range
  • Supports nitrocellulose and PVDF membranes
  • Compatible with goat anti-rabbit, anti-mouse or streptavidin labeled antibodies
  • Reads membranes fitting the ScanLater SpectraMax Membrane Holder

Figure 1. ScanLater™ Western Blot Assay Workflow

ScanLater Assay principle rev B 430px.png
Figure 1. The ScanLater System simplifies the traditional western blot protocol by employing a secondary antibody labeled with a europium-chelate. The blot is then read using the ScanLater Western Blot Cartridge on the SpectraMax® i3 or Paradigm Multi-Mode Platforms. No substrates are needed and the blot can be scanned immediately after washing.

Figure 2. ScanLater Western Blot Assay Principle

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Figure 2. Schematic of protein detection method shows the secondary antibody is directly labeled with Europium.

Figure 3. Europium-chelate excitation and emission spectra

Fig 1a(0).png

Figure 4. Schematic representation of emission lifetimes that minimize background fluorescence

ScanLater Fig. 1b.jpg
Figure 4. Schematic representation of emission lifetimes of background fluorescence and Eu-chelate showing principle of TRF measurement. Signal is measured after appropriate time-delay to reduce auto-fluorescence.

 

Representative applications: 

  • Protein identification and quantitation
  • Epitope mapping
  • Amino acid composition and sequence analysis
  • Spots imprinting
  • Phosphoprotein endogenous or exogenous expression
  • Protein resillience
  • Structure domain analysis
  • Protein expression
  • Protein content in serum
  • QC elimination of albumin and lgG in serum
  • Protein expression the cell cycle

ScanLater Western Blot System sensitivity and dynamic range

ScanLater Fig. 2.jpg
Figure 1. Image of Gluthathione S-transferase dilution series as scanned by the SpectraMax Paradigm Reader. Note: False-color scale is used to show large dynamic range. Integrated intensities from individual bands showing total dynamic range of greater than 3 logs.

Comparison of ScanLater Western Blot System to chemiluminescent western blot detection

ScanLater Fig. 5.jpg
Figure 2. Detection of ubiquitinated Rad-18 in HEK 293 cells on treatment with different concentration (0, 50, 100 ppm) of carcinogen MMS, an alkylating agent. Comparable result using ScanLater Western Blot System vs. chemiluminescence western blot (Stanford University).

 

The ScanLater Western Blot Detection Cartridge is compatible with the SpectraMax i3 and SpectraMax Paradigm Multi-Mode Detection Platforms. For more information, click on the link to the Molecular Devices Multi-Mode Microplate Reader Comparison Table.

Related Video

Learn how you can run your western blots on a microplate reader in this video featuring the ScanLater Western Blot Detection System.
 

 

Related Collateral

Brochure

Data Sheet

Application

Poster

 

Product Name Description Part Number
System
ScanLater Western 
Blot Detection System
  • Membrane sample holder
  • SoftMax Pro Software 6.3.1
  • Image plug-in for data analysis
0200-7027
Assay Kit
ScanLater: Eu-Streptavidin
Evaluation Kit
  • Europium-labeled streptavidin (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8200

Click here to purchase
Assay Kit
ScanLater: Eu-Streptavidin
Bulk Kit
  • Europium-labeled streptavidin (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8203

Click here to purchase
Assay Kit
ScanLater: Anti-Mouse
Evaluation Kit
  • Europium-labeled goat anti-mouse antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8201

Click here to purchase
Assay Kit
ScanLater: Anti-Mouse
Bulk Kit
  • Europium-labeled goat anti-mouse antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8205

Click here to purchase
Assay Kit
ScanLater: Anti-Rabbit
Evaluation Kit
  • Europium-labeled goat anti-rabbit antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8202

Click here to purchase
Assay Kit
ScanLater: Anti-Rabbit
Bulk Kit
  • Europium-labeled goat anti-rabbit antibody (60 μg)
  • Blocking buffer (120 mL), Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8204

Click here to purchase
Assay Kit
ScanLater: Anti-Goat
Evaluation Kit
  • Europium-labeled donkey anti-goat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8224

Click here to purchase
Assay Kit
ScanLater: Anti-Goat
Bulk Kit
  • Europium-labeled donkey anti-goat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8227

Click here to purchase
Assay Kit
ScanLater: Anti-Rat
Evaluation Kit
  • Europium-labeled donkey anti-rat antibody (20 μg)
  • Blocking buffer (40 mL)
  • Wash buffer (100 mL)
  • For 10 mini gels with recommended protocol
R8225

Click here to purchase
Assay Kit
ScanLater: Anti-Rat
Bulk Kit
  • Europium-labeled donkey anti-rat antibody (60 μg)
  • Blocking buffer (120 mL)
  • Wash buffer (300 mL)
  • For 30 mini gels with recommended protocol
R8228

Click here to purchase
Protein Ladder
ScanLater Western Blot Protein Ladder
  • 7 biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD
  • 3 pre-stained marker proteins at 18, 31, and 70 kD
  • Ready to use, for 30 mini gels with recommended
    protocol
R8220

Click here to purchase
Assay Kit Components
ScanLater Wash Buffer (100 mL) R8206
ScanLater 5x Blocking Buffer (120 mL) R8207
ScanLater Goat Anti-Mouse Eu-labeled Antibody (60 µg) R8208
ScanLater Goat Anti-Rabbit Eu-labeled Antibody (60 µg) R8209
ScanLater Eu-labeled Antibody Streptavidin (60 µg) R8212
ScanLater Eu-Anti-Goat IgG (60 μg) R8226
ScanLater Eu-Anti-Rat IgG (60 μg) R8229