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Comparison of Microplate Readers with Luminescence Detection

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SpectraMax i3x Multi-Mode Detection PlatformSpectraMax Paradigm Multi-Mode Microplate ReaderSpectraMax M Series Multi-Mode Microplate ReadersFlexStation 3 Multi-Mode Microplate ReaderSpectraMax L Microplate ReaderFilterMax F3 & F5 Multi-Mode Microplate Readers
DetectorPhotomultiplier tubes (PMT) and/or photodiode2 PMTPhoto-multiplier tube2 PMT1, or 2 Ultra-fast Photomultiplier TubesSilicon Photodiode + PMT
Spectral range300-850 nm300-850 nm250-850 nm250-850 nm380-630 nmF3: 400 - 650nm
FilterMax F5: 400 - 750 nm
Dynamic range> 6 decades> 6 decades> 6 decades> 6 decades> 9 decades6 decades
Sensitivity3 pM for 96-wells, 6 pM for 384-wells (Optimized for ATP-Glow)1.25 pM for 96-wells, 3 pM for 384-wells, 19 pM for 1536-wells (Optimized for ATP-Glow)43 pM ATP in 96 wells (optimized)<2 fg/well for firefly luciferase in 96- and 384-well top read< 20 attomol ATP per well
Microplate format6 to 1536 wells*6- to 1536-wells6-384-well microplates6-384 (96- and 384-wells with pipetting)96- and 384-well microplatesF3: 96 - 384 well
F5: 6 - 1536 well
Cross-talk< 0.4% in white 96- and 384-well microplates<0.3% in white 96- and 384-well microplates<0.3% in white 96- and 384-well microplates< 3 x 10-5 (96-well)
Read modesSpectral, Endpoint, KineticEndpoint, KineticEndpoint, KineticEndpoint, Kinetic, Fast KineticEndpoint, Kinetic, Fast Kinetic, Dual-readEndpoint, Kinetic, Linear Scan, Area Scan
Read times0:30 minutes for 96-wells, 1:15 minutes for 384-wells2:00 minutes for 96-wells, 7:00 minutes for 384-wells2:00 minutes for 96-wells, 7:00 minutes for 384-wells0.1 up to 3600 sec. in steps of 0.1 sec.18sec/96well
37sec/384well
Injectors/pipettorsOption availableOption available8- or 16-channel pipettor2 variable volume injectors
Injection/pipettor volumesMax. volume 200uL for 8-channel and 30 uL for 16-channel
Temperature control2 degrees C above ambient to 45 degrees C2 degrees C above ambient to 45 degrees C2 degrees C above ambient to 60 degrees C2 degrees C above ambient to 45 degrees C5 degrees C above ambient to 45 degrees C
SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro SoftwareSoftMax Pro Software
Validation & compliance toolsIQ/OQ Protocols, SoftMax Pro GxP Compliance Software, SpectraTest LM1 Validation PlateSoftMax Pro GxP Compliance SoftwareIQ/OQ Protocols, FDA 21 CFR Part 11 Compliance, SpectraTest LM1 Validation PlateIQ/OQ/PQ Protocols, FDA 21 CFR Part 11 ComplianceSoftware Validation Package, IQ/OQ Documentation, Physical Validation Test Plate
Robotic compatible

* 1536 detection available via detection cartridges