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MultiClamp 700B Amplifier

For single-channel recordings, whole-cell voltage- and current-clamp recordings, intracellular sharp electrode recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies

A microelectrode amplifier that combines leading edge electronics design and a wealth of features and functions with an intuitive user interface that simplifies the creation of complex protocols.

​The MultiClamp™ 700B Microelectrode Amplifier is a versatile, computer-controlled microelectrode amplifier designed for patch voltage-clamp (resistive-feedback, four ranges) or high-speed current clamp (voltage-follower, three ranges) recording within the same headstage. Many automated functions and effective signal conditioning. Ideal for many applications, including high-speed current clamp (sharp-electrode or field potentials), patch clamp (whole-cell, macro-patch, or excised patch), voltammetry/amperometry, ion-selective measurements and bilayer recordings. Add two more optional headstage (voltage-follower) inputs for a four-point recording system, ideal for complex synaptic recordings. Major new features of the MultiClamp 700B Amplifier include Internal Automatic Mode switching, triggered by external signals or the recording itself; Oscillation Suppression for better cell protection; and Slow Current Injection to compensate for drifting potentials.

  • Support of up to four headstages makes the MultiClamp 700B System four amplifiers in one.
  • Software control of all amplifier settings for automated optimization features, reduction of manipulation steps, and convenient, reliable telegraphing.
  • Quick Select Feature recalls up to three saved amplifier configurations for fast, reproducible application switching.
  • Optional SoftPanel allows amplifier control using knobs and buttons for a more conventional feel to amplifier control.
  • Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters.
  • Dual Command Potentials add flexibility by processing input signals from two different sources.
  • Selectable command sensitivity for flexible control of the output waveform.
  • Pipette offset compensation with auto-mode quickly removes signal offsets.
  • Automatic pipette capacitance compensation requires only the push of a button to neutralize the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry.
  • Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential.
  • Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance.
  • Automatic cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings with a single mouse click.
  • Automatic mode switching enables you to control the transition between voltage and current clamp by an external trigger or depending on the recorded signal.
  • Automatic oscillation detection prevents damage to a cell when electrical feedback occurs.
  • Leak subtraction for automatic correction of leak currents with a smart, software-based algorithm.
  • Selectable output gain with 11 settings to scale the output signal to the desired level.
  • Audio monitor for acoustic tracking of the signal while you are busy looking down a microscope.
  • Fine-tunable 4-pole bessel and butterworth low-pass filters act as anti-aliasing filters and can be used to pre-condition the output signal.

 

Basic Ion Channel Research

Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Learn more.

Cell Pathway Analysis

Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. Learn more.

Disease Research

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease. Learn more.

Safety Assessment

A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Learn more.

MultiClamp Commander

MultiClamp Comander Screen shot
MultiClamp Commander

Instead of the traditional front panel knobs and switches, the MultiClamp 700B Amplifier is controlled by the MultiClamp 700B Commander, a software program that runs on a host computer (PC and Macintosh; see System Requirements) and communicates with the amplifier via a USB interface. This control interface reports resistance, voltage, and current measurements and provides automation of Bridge Balance, Pipette Offset, and Pipette Capacitance Neutralization for improved ease of use.  Amplifier settings such as gain, filter frequency, whole-cell capacitance, recording mode, and input/output scale factors are automatically telegraphed to the data acquisition software via "messaging" through the computer operating system.  Quick Select provides easy access to stored preset configurations that apply to particular applications.

In addition, MultiClamp Commander has "smart" features that protect cells from potentially damaging signal oscillations and automatic mode changes based on internal signal thresholds or externally applied signals. Amplifier settings such as gain, filter frequency, recording mode, and input/output scale factors are automatically telegraphed to the pCLAMP 10 Data Acquisition Software via the USB connection. This ensures that the amplifier settings are accurately and conveniently stored in the recorded data files.

To obtain the installer for a more in-depth look at MultiClamp 700B Commander Software, please go to our Customer Support page.

In combination with a Digidata® 1550B Data Acquisition System and pCLAMP™ Software, the MultiClamp 700B Amplifier forms the core of a setup like those used in many electrophysiology labs around the world. Computer control of the amplifier ensures correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the features of the MultiClamp 700B Amplifier.

Developer Support

The MultiClamp Software Development Kit provides the tools for programmers who wish to develop software that is capable of collecting information about the state of the MultiClamp 700B or 700A Amplifier (i.e., telegraph settings). This will be useful for Microsoft Windows software development, since the applications will communicate via a registered messaging interface.

The latest version of MultiClamp 700 Commander automatically installs the files required for 3rd party support. Browse the "\moleculardevices\multiclamp-commander" directory to locate the files.

Note: Although we are committed to the integrity of this SDK, Molecular Devices will not provide direct technical support for writing programs using the SDK.

General Specifications

General Specifications
Dimensions (in.) 3.5 (H)x 19 (W) x 12 (D)
Dimensions (cm) 8.9 H) x 48.3 (W) x 30.5 (D)
Weight 10 lbs. (4.54 kg)
Headstage (in.) 0.875 (H) x 1.625(W) x 2.3125 (D)
Headstage (cm) 2.0 (H) x 4.0 (W) x 8.4 (D)
Channels 2 (sharing a common ground)
Communications USB 1 Type B female ports
Rack use Standard 19" rack-mount (2U) with handles
Benchtop use Bayonet feet
Power
85-260 VAC
50-60 Hz, 30 W (max)
Safety CE marking (Conformité Européenne)

Detail Specifications

Configurations

The MultiClamp 700B comes with two identical but independent CV-7B standard headstages, each of which contains both current-to-voltage and voltage-following circuitry. This design allows the user to rapidly switch between patch clamp recording and true high-speed current clamp recording. Thus, with two headstages, the MultiClamp 700B can perform the function of two patch clamps, two current clamps, or a combination of patch and current clamp amplifiers. Furthermore, two optional voltage-follower headstages (HS-2 type) can be connected to auxiliary inputs to allow third and fourth-point voltage recording.
 
HS-2 and HS-2A unity-gain headstages
The HS-2 and HS-2A headstages are used with Axoclamp 2 and GeneClamp amplifiers. They are all unity-gain voltage recording headstages but they come in a variety of different current-passing gains for applications as diverse as extracellular recording, bath-potential recording, ion-sensitive recording, ionophoresis and intracellular recording from small or large cells. For ultra-high impedance electrodes, special circuitry inside the headstage prevents any DC current from leaking into the input through the capacitance neutralization circuit.
  • HS-2-x0.0001MU
  • HS-2-x0.01MU
  • HS-2A-x0.1LU
  • HS-2A-x1LU
  • HS-2A-x1MGU
  • HS-2A-x10MGU
  • HS-2A-x100MGU
  • VG-2A-x100 (virtual ground)
 
The CV-7B was designed for low noise and flexible recording features. However, some specialized applications require even more flexibility. For example, the rather large membrane capacitance in bilayer recording demands greater capacitance compensation. The optional CV-7B/BL headstage was developed for this purpose. Another optional headstage, the CV-7B/EC, was designed for large (± 2 V) commands required during electrochemistry recording (amperometry, voltammetry).
 
Each of the two standard CV-7B headstages can be replaced with a headstage for a specialized application.  Available headstages are,
  • CV-7B/BL for lipid bilayer recordings
  • CV-7B/EC for electrochemistry recordings (amperometry, voltammetry)
MultiClamp 700B Headstages
Description Details Part number
MultiClamp 700B Headstage CV-7B Patch-clamp headstage 1-CV-7B
MultiClamp 700B Headstage CV-7B/BL Bilayer headstage 1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC Electrochemistry headstage 1-CV-7B/EC

 

Headstages

Description Details Part Number
Axoclamp 900A Headstage HS-9A X0.1U x0.1 headstage 1-2950-0359
Axoclamp 900A Headstage HS-9A X1U x1 headstage 1-2950-0360
Axoclamp 900A Headstage HS-9A X10U x10 headstage 1-2950-0361
Axoclamp 900A Headstage VG-9A X10U x10 virtual ground headstage 1-2950-0362
Axoclamp 900A Headstage VG-9A X100U x100 virtual ground headstage 1-2950-0363
MultiClamp 700B Headstage CV-7B Patch-clamp headstage 1-CV-7B
MultiClamp 700B Headstage CV-7B/BL Bilayer headstage 1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC Electrochemistry headstage 1-CV-7B/EC

Electrode holders, adapters, and holder components

Description Details Part Number
Electrode Holder for U-Type Headstages Fits glass pipettes with outer diameter of 1.0 - 1.7 mm 1-HL-U
Electrode Holder Replacement Caps Set of 2 polycarbonate caps for HL-U holders 1-HL-CAP
Cone Washers 1.1mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm 1-HLC-11
Cone Washers 1.3mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm 1-HLC-13
Cone Washers 1.5mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm 1-HLC-15
Cone Washers 1.7mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm 1-HLC-17
Pins 1mm for HL-U Holders Set of 3 brass pins for HL-U holders, 1mm 1-HLP-U
2mm Plugs with Solder Cups Set of 5 general purpose gold plugs, 2mm, with solder cups 1-HLP-0
Silver Wire Set of 5 Ag wires, 0.25mm diameter, 50mm long 1-HLA-005
Silicone Tubing for Silver Wire 1mm ID x 70mm Long Silicone Tubing 1-HLT-70
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assemblies 1-HLA-003
Adapter For BNC Holders To U-Type Headstages Connects BNC holders to CV and HS headstages with threaded collets (U-type) 1-HLB-U
Right-Angle Adapter for HL-U Electrode Holders Fits CV and HS headstages with threaded collets (U-type) 1-HLR-U

Model Cells

Description Details Part Number
Model Cell for Oocytes Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages 1-MCO-2U
Model Cell for TEVC / DSEVC Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages 1-CLAMP-1U
Model Cell for Whole Cell/ Single Channels Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages 1-PATCH-1U
Model Cell for Bilayers Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages 1-MCB-1U

Cables

Description Details Part Number
Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers 1-2100-0934

Miscellaneous

Description Details Part Number
SoftPanel Amplifier Control Unit Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection. 1-SOFTPANEL (USB)
Remote Buzz For Axoclamp 900A Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms) 1-2950-0366
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assembly and Ag wire 1-HLA-003

Number of Citations*: 7,520

Latest Citations:

For a complete list of citations, please click here.

 

Tonic Firing Rate Controls Dendritic Ca2+ Signaling and Synaptic Gain in Substantia Nigra Dopamine Neurons

TA Hage, ZM Khaliq - The Journal of Neuroscience, 2015 - Soc Neuroscience
... cell recordings, broad APs (halfwidth >1.35 ms) and prominent voltage sag in response to negative
current injection, associated with hyperpolarization-activated cation current (I H ). Current-clamp
and voltage-clamp recordings were made with a Multiclamp 700B amplifier and ...

The Parkinson's Disease-Associated Mutation LRRK2-G2019S Impairs Synaptic Plasticity in Mouse Hippocampus

ES Sweet, B Saunier-Rebori, Z Yue… - The Journal of …, 2015 - Soc Neuroscience
... Recordings were acquired with a Multiclamp 700B and Digidata 1440A (Molecular Devices). ...
Recordings were obtained in submersion chambers with room temperature ACSF using
Multiclamp 700B and Digidata 1440A (Molecular Devices). ...
 

The Contribution of Raised Intraneuronal Chloride to Epileptic Network Activity

H Alfonsa, EM Merricks, NK Codadu… - The Journal of …, 2015 - Soc Neuroscience
... Electrophysiological data were collected using Multiclamp 700B (Molecular Devices)
and Digidata acquisition boards connected to desktop computers (Dell Computer
Company) running pClamp software (Molecular Devices). ...
 

Nicotinic Receptor Subtype-Selective Circuit Patterns in the Subthalamic Nucleus

C Xiao, JM Miwa, BJ Henderson, Y Wang… - The Journal of …, 2015 - Soc Neuroscience
... Single or dual whole-cell patch-clamp techniques were used to record electrophysiological signals
from neurons with MultiClamp 700B amplifiers (Molecular Devices), Digidata 1322
analog-to-digital converters (Molecular Devices), and pClamp 9.2 software (Molecular Devices ...
 

Functional Diversity of Subicular Principal Cells during Hippocampal Ripples

C Böhm, Y Peng, N Maier, J Winterer… - The Journal of …, 2015 - Soc Neuroscience
... All in vivo signals were amplified with a Multiclamp 700B (Molecular Devices), filtered at 10 kHz,
and digitized at 20 kHz (ITC-18; HEKA Elektronik). ... The pH was adjusted to 7.20 with KOH.
Recordings were performed using Multiclamp 700B amplifiers (Molecular Devices). ...

Synaptic Function of Rab11Fip5: Selective Requirement for Hippocampal Long-Term Depression

T Bacaj, M AhmadS Jurado… - The Journal of …, 2015 - Soc Neuroscience
... Synaptic currents were monitored with a Multiclamp 700B amplifier (Molecular Devices). ...
Data were collected with a MultiClamp 700B amplifier (Molecular Devices) and digitized
at 10 kHz using the A/D converter ITC-18 (Instrutech). ...

Potent KCNQ2/3-specific channel activator suppresses in vivo epileptic activity and prevents the development of tinnitus

BI KalappaH Soh, KM Duignan, T Furuya… - The Journal of …, 2015 - Soc Neuroscience
... Data were acquired through a Multiclamp 700B amplifier (Molecular Devices), low-pass filtered
at 2 kHz, and sampled at 10 kHz. Constructs. ... Current responses were collected with a Multiclamp
700B amplifier (Molecular Devices), filtered at 2 kHz, and sampled at 10 kHz. ...
 

Muscarinic Receptors Modulate Dendrodendritic Inhibitory Synapses to Sculpt Glomerular Output

S Liu, Z Shao, A Puche, M Wachowiak… - The Journal of …, 2015 - Soc Neuroscience
... Current or voltage signals were recorded with a MultiClamp 700B Amplifier (Molecular Devices),
and low-pass filtered at 4 kHz and sampled at 10 kHz with a DIGIDATA 1322A 16 bit
analog-to-digital converter (Molecular Devices) using Clampex version 9.2 (Molecular Devices ...

Kisspeptin regulation of arcuate neuron excitability in kisspeptin receptor knockout mice

X Liu, A Herbison - Endocrinology, 2015 - press.endocrine.org
... Signals (voltage and current) were amplified with a Multiclamp 700B amplifier (CV7B;
Molecular Devices) and sampled on-line with the use of a Digidata 1440A interface
(Molecular Devices) connected to a personal computer. ...
 

Effect of-Allocryptopine on Delayed Afterdepolarizations and Triggered Activities in Mice Cardiomyocytes Treated with Isoproterenol

B Xu, Y Fu, L Liu, K Lin, X Zhao… - Evidence-Based …, 2015 - downloads.hindawi.com
... Transmembrane action potentials and currents were recorded in whole cell configuration as previ-
ously described using a MultiClamp 700B amplifier (Axon Instruments) [7]. Correction for liquid
junction potentials (which averaged −10 mV) was applied only for resting poten- tial ...

 

* as of November 13, 2015. Source: Google Scholar.  Search results include "Multiclamp 700B" and "Multiclamp Axon".