For a variety of electrophysiology recordings including single-channel recordings, whole-cell voltage- and current-clamp recordings, intracellular sharp electrode recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies.
The Axon Digidata® System is the most widely used for a wide variety of electrophysiology recordings in laboratories worldwide. The Digidata 1550 Digitizer further extends the precision and quality of the existing Axon Digitizer platform with enhanced performance in command voltage output control and sampling rate. It enables the recording of up to 8 cells simultaneously to study the function of neuronal networks in your neuroscience research. It also achieves high-resolution data acquisition by capturing more data points in less time in your ion channel research.
Recordings from Axon Digidata 1550 System
The Axon Digidata® 1550 Digitizer is a low-noise high-resolution 16-bit data acquisition system for electrophysiology recordings. It is self-contained and communicates with the host computer via a USB 2.0 interface for extremely easy installation and set-up. Designed for fast results, the Digidata 1550 Digitizer comes with AxoScope software for Microsoft Windows OS and is ready to acquire data immediately after installation. Absolutely no programming is necessary.
The Digidata 1550 Digitizer features a maximum sampling rate of 500 kHz per channel, with an outstanding total data throughput rate of 4 mega-samples per second. This enables you to capture even the fastest signals in the biorelevant frequency range. Both the inherent digitizer noise and channel crosstalk noise are rated at less than ±1 mV average p-p at 10 kHz, within a ±10 V input range, making the Digidata 1550 System one of the quietest digitizers on the market.
For intuitive set-up, the front panel is clearly laid out with eight analog input channels and eight analog output channels, eight general digital outputs, one dedicated digital output to trigger devices such as oscilloscopes, as well as trigger inputs to start acquisition and to tag data. The back panel has four additional analog instrument telegraph inputs, as well as a DB-25 connector for the digital outputs. With its USB 2.0 interface, you can easily connect the Digidata 1550 Digitizer to a laptop computer without the need for a peripheral PC card.
Designed to support continuous data acquisition within a multitasking operating system, the Digidata 1550 Digitizer is fully compatible with the included AxoScope 10.5 Software for Microsoft Windows 7 (32-bit or 64-bit) and the optional pCLAMP Software for Windows.
Basic Ion Channel Research
Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Learn more.
Cell Pathway Analysis
Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. Learn more.
Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease. Learn more.
A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Learn more.
AxoScope 10.5 Software
AxoScope 10.5 Software is a turn-key data acquisition and analysis program for Microsoft Windows, designed to replace oscilloscopes, chart recorders, and FM tape recorders. AxoScope 10.5 Software provides from eight to sixteen channels of analog acquisition and four different acquisition modes.
Data can be acquired and displayed continuously in Gap-Free mode up to the supported sampling rate of the digitizer. Other acquisition modes supported by AxoScope 10.5 Software are threshold-triggered Fixed-Length Events, Variable-Length Events or High-Speed Oscilloscope modes. Tags and comments can be added to the data in real time. Analog and digital output holding values can be altered.
AxoScope 10.5 Software opens Axon-format ABF data files and enables quick analysis of sections of interest with an array of browsing and basic analysis tools. Data and page layout can be reviewed before printing. Additional features include voice tags, which allow tagging of data with spoken comments*, low-pass and high-pass digital filtering of incoming data, and Store Trace, which freezes a snapshot of a waveform on the screen for comparison with a subsequent input.
In combination with pCLAMP 10.5 Software, the Digidata® 1550 System forms the data acquisition backbone of a set-up like those used in many electrophysiology labs around the world. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the features of the Digidata 1550 Low-Noise Data Acquisition System.
* Requires a sound chip in the host computer and a microphone
Software testbeds are available to help programmers write data acquisition programs for Digidata Digitizers. These testbeds include sample code and DLL drivers for Windows programs. For non-Windows programs, it is suggested that you write a C wrapper for our API.
There are three testbeds currently available through our Customer Support page:
- The Digidata 1550 Testbed allows programs to be created to control the Digidata 1550 Digitizer. The sample application requires a Digidata 1550 Digitizer to run.
- The Digidata 1440A Testbed allows programs to be created to control the Digidata 1440A Digitizer. The sample application requires a Digidata 1440A Digitizer to run.
- The Digidata 132x Testbed allows programs to be created to control the product line of Digidata 1320A, 1321A and 1322A digitizers. Note that the Adaptec WinASPI layer is also required.
Please note that technical support is not available for writing programs using these testbeds.
- 8 analog input channels to digitize acquired signals up to 500 kHz independently
- 8 analog output channels to send command voltage output independently
- 8 digital out channels to control the periphery equipment used in sophisticated experiments
- Independent analog-to-digital converters for each input channel ensure low crosstalk levels and high data acquisition rates.
- USB 2.0 connection allows operation on virtually any current PC computer running Microsoft Windows 7 (32-bit or 64-bit) Operating System, including laptops.
- 16-bit resolution data
- State-of-the-art signal-to-noise ratio
- All signal connectors on the front panel for ease of access and maintenance of the electrophysiology set-up
- Rack-mount case for tidy installation of all components in the electrophysiology set-up
- User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference.
|Number of input channels||8 single-ended|
|Number of ADCs||8|
1 Hz - 500 kHz; Maximum aggregate throughput rate is 500 kHz x 8 input channels = 4 Megasamples/s
|Input range||-10.000 V to +10.000 V|
|Input resistance||>1 MΩ|
|Number of channels||8|
|Number of DACs||8|
|Sampling rates||1 Hz - 500 kHz|
|Output range||-10.000 V to +10.000 V|
|Output impedance||< 0.5 Ω|
|Output short circuit to signal ground||±25 mA|
|Number of bits||8 (of 16) supported in software|
|SCOPE||Dedicated trigger output|
|Output driver||Advanced CMOS (AC) compatible|
|Output current||±4 mA source, cable|
|Type||USB 2.0 braided|
|Input type||TTL compatible|
- Axopatch 200B Amplifier Brochure
- MultiClamp 700B Amplifier Datasheet
- Axoclamp 900A Amplifier Datasheet
- Digidata 1550 Digitizer Datasheet
- pCLAMP Datasheet
|Axoclamp 900A Headstage HS-9A X0.1U||x0.1 headstage||1-2950-0359|
|Axoclamp 900A Headstage HS-9A X1U||x1 headstage||1-2950-0360|
|Axoclamp 900A Headstage HS-9A X10U||x10 headstage||1-2950-0361|
|Axoclamp 900A Headstage VG-9A X10U||x10 virtual ground headstage||1-2950-0362|
|Axoclamp 900A Headstage VG-9A X100U||x100 virtual ground headstage||1-2950-0363|
|MultiClamp 700B Headstage CV-7B||Patch-clamp headstage||1-CV-7B|
|MultiClamp 700B Headstage CV-7B/BL||Bilayer headstage||1-CV-7B/BL|
|MultiClamp 700B Headstage CV-7B/EC||Electrochemistry headstage||1-CV-7B/EC|
Electrode holders, adapters, and holder components
|Electrode Holder for U-Type Headstages||Fits glass pipettes with outer diameter of 1.0 - 1.7 mm||1-HL-U|
|Electrode Holder Replacement Caps||Set of 2 polycarbonate caps for HL-U holders||1-HL-CAP|
|Cone Washers 1.1mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm||1-HLC-11|
|Cone Washers 1.3mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm||1-HLC-13|
|Cone Washers 1.5mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm||1-HLC-15|
|Cone Washers 1.7mm ID||Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm||1-HLC-17|
|Pins 1mm for HL-U Holders||Set of 3 brass pins for HL-U holders, 1mm||1-HLP-U|
|2mm Plugs with Solder Cups||Set of 5 general purpose gold plugs, 2mm, with solder cups||1-HLP-0|
|Silver Wire||Set of 5 Ag wires, 0.25mm diameter, 50mm long||1-HLA-005|
|Silicone Tubing for Silver Wire||1mm ID x 70mm Long Silicone Tubing||1-HLT-70|
|Silver/Silver Chloride Pellet Assemblies||Set of 3 Ag/AgCl pellet assemblies||1-HLA-003|
|Adapter For BNC Holders To U-Type Headstages||Connects BNC holders to CV and HS headstages with threaded collets (U-type)||1-HLB-U|
|Right-Angle Adapter for HL-U Electrode Holders||Fits CV and HS headstages with threaded collets (U-type)||1-HLR-U|
|Model Cell for Oocytes||Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages||1-MCO-2U|
|Model Cell for TEVC / DSEVC||Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages||1-CLAMP-1U|
|Model Cell for Whole Cell/ Single Channels||Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages||1-PATCH-1U|
|Model Cell for Bilayers||Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages||1-MCB-1U|
|Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier||Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers||1-2100-0934|
|SoftPanel Amplifier Control Unit||Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection.||1-SOFTPANEL (USB)|
|Remote Buzz For Axoclamp 900A||Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms)||1-2950-0366|
|Silver/Silver Chloride Pellet Assemblies||Set of 3 Ag/AgCl pellet assembly and Ag wire||1-HLA-003|