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Axoporator 800A

The world's first commercially available single-cell electroporator represents the convergence of biophysics, cell biology, and molecular biology.

The world's first commercially available single-cell electroporator represents the convergence of biophysics, cell biology, and molecular biology. 

The Axoporator® 800A Electroporation System, a dedicated single-cell electroporation system that makes it possible to electroporate individually targeted cells. Provides precise control of voltage pulse and train parameters, resulting in optimized targeted delivery of ions or small molecules into a select number of cells in culture, in tissue or in vivo.

  • Ready to use. No computer is necessary. A microscope, a micromanipulator, and a source of micropipettes are everything needed to complete the system.Small footprint for easy positioning near the microscope
  • Three different pulse types, configurable in either polarity, ensure the optimal waveform for your application:
  • Rectangular "square"
  • Bipolar
  • Bilevel: a special pulse that has been demonstrated to effectively and efficiently deliver genes as large as 14 kb into a cell
  • Pulse trains for greatest flexibility of the voltage waveform
  • Automatic and manual counters simplify tracking the number of electroporation commands.
  • Voltage range up to 100 V provides the optimal voltage for single-cell electroporation, which can be up to several tens of volts in some preparations.
  • Available AP-1A-0.1MU headstage with current passing range up to ±100 μA
  • Built-in audio monitor with headphone connector changes tone as you near a cell and make contact.
  • Resistance display, in combination with audio monitor, provides a useful tool for searching out cells in a tissue slice without visual control
  • Stand-alone or computer-controlled pulse start for greatest flexibility and synchronization with electrophysiology experiments.  See Related Products section for more details
  • Foot switch to trigger pulse start while your hands are busy
  • Sync output BNC for synchronization of a third-party recording system
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

Electroporation is a common technique used in molecular biology laboratories to introduce polar or charged molecules, such as drugs, proteins, peptides, or genes, into a cell.  The Axoporator® 800A Single-Cell Electroporation System represents the convergence of biophysics, cell biology, and molecular biology. If you are already performing electrophysiology recordings or visualization experiments in your lab, you probably have everything that is required to add the Axoporator 800A Electroporator System to your setup.

Documented Success

The Axoporator 800A Electroporator System was developed with advice from, and tested by, the pioneers in single-cell electroporation. A collection of their successful protocols are documented in the user manual.

Cell Types Electroporated

Some of the cell types that have been electroporated by Axoporator 800A Single-Cell Electroporation System are listed here.  Listed with each cell type are the compounds successfully delivered.

Aplysia Neurons

Rat Hippocampal Pyramidal Neurons in Acute Slice

  • FITC dextran 3000
  • Tetramethylrhodamine dextran 3000

Mouse Cortex Neurons in Acute Slice

  • FITC dextran 3000
  • Tetramethylrhodamine dextran 3000

Rat Dorsal Root Ganglion Cells

  • Alexa 488
  • Alexa 568
  • Clontech dsRed 1 mitochondrial clone
  • Clontech eGFP peroxisome clone
  • Dextran FITC 4,10,20,40,70,250

Aplysia Gastric Ganglia

  • FITC dextran 3000

Rat Retinal Ganglion Cells

  • Alexa 488
  • Alexa 568
  • Clontech dsRed 1 mitochondrial clone
  • Clontech eGFP peroxisome clone
  • Clontech eCFP nuclear clone
  • Dextran FITC 4,10,20,40,70,250

Rat Photoreceptors

  • Alexa 488
  • Alexa 568

Zebrafish Tectal Neurons

  • pEGFP
  • Alpha TN4 Cells (a mouse lens epithelium clonal cell line)
  • Alexa 488
  • Alexa 568
  • Clontech dsRed 1 mitochondrial clone
  • Clontech eGFP peroxisome clone
  • Clontech eCFP nuclear clone
  • Dextran FITC 4,10,20,40,70,250
  • eGFP vimentin
  • eGFP actin
  • eGFP Kir2.1 inward rectifier clone
  • eGFP Kv2.1 delayed rectifier clone
  • eGFP Bk alpha subunit clone
  • 40 eGFP partial sequence clones of Kir2.1
  • CaCl2
  • 2,4-Dinitrophenol
  • Cytochalasin D
  • eGFP, eCFP, eYFP or dsRed 1 clones of CD-20, adaptin, golgi, tubulin, endoplasmic reticulum, Lamin B, and lysosome
  • siRNA's of several kinds

Rat Conjunctival Epithelium

  • Alexa 488

Rat Ciliary Epithelium

  • Alexa 488

Rat Lens Epithelium (collagenase treated to remove capsule)

  • Alexa 488
  • Alexa 568

Rat Corneal Endothelium

  • Alexa 488
  • Alexa 568

Rat Corneal Epithelium

  • Alexa 488

Human Lens Epithelium (decapsulated prep)

  • Alexa 488
  • Alexa 568

Human Lens Epithelium (cultured)

  • Alexa 488
  • Clontech eCFP nucleus clone
  • Clontech eYFP peroxisome clone
  • Clontech dsRed1 mitochondrial clone
  • Dextran FITC 4,10,20,40,70,250

Bovine Corneal Epithelium (cultured)

  • Alexa 488
  • Clontech eCFP nuclear clone
  • Clontech eYFP peroxisome clone
  • Clontech dsRed1 mitochondrial clone

Guinea Pig Interstitial Cells of Cajal

  • Alexa 488
  • Alexa 568
  • Clontech dsRed1 mitochondrial clone
  • Clontech eGFP peroxisome clone
  • Dextran FITC 4,10,20,40,70,250

The Axoporator® 800A Electroporator System was designed as a turn-key, stand-alone system and does not require dedicated control software. However, cooperation with separately available pCLAMP Software and the Digidata 1550B Data Acquisition System is supported. See the Related Products section for more details.

General Specifications

  • Dimensions (in.): 4.1 (H) x 10.3 (W) x 7.3 (D)
  • Dimensions (cm): 10.5 (H) x 26.2 (W) x 18.5 (D)
  • Weight: 3.2 lbs. (1.45 kg)
  • Power*: 100-264 VAC, max 0.9A, 50-60 Hz
  • DC to main unit: 12-14 VDC

* Using the provided power supply

Headstage Specifications

  • Dimensions (in.): 2.3 (H) x 1.1 (W) x 0.9 (D)
  • Dimensions (cm): 5.7 (H) x 2.9 (W) x 2.2 (D)

More detailed specifications are listed in the User Guide for the Axoporator® 800A Electroporator.  To obtain the User Guide, please go to our Customer Support page.

Headstages

Description Details Part Number
Axoporator 800A Headstage AP-1A X0.1MU x0.1MU headstage, 0.1 M Rf 1-2950-0306
Axoporator 800A Headstage AP-1A X1MU x1MU headstage, 1 M Rf 1-2950-0305

Electrode Holders, Adapters, and Holder Components

Description Details Part Number
Electrode Holder for U-Type Headstages Fits glass pipettes with outer diameter of 1.0-1.7mm 1-HL-U
Electrode Holder Replacement Caps Set of 2 polycarbonate caps for HL-U holders 1-HL-CAP
Cone Washers 1.1mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0-1.1mm 1-HLC-11
Cone Washers 1.3mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1-1.3mm 1-HLC-13
Cone Washers 1.5mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3-1.5mm 1-HLC-15
Cone Washers 1.7mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5-1.7mm 1-HLC-17
Pins 1mm for HL-U Holders Set of 3 brass pins for HL-U holders, 1mm 1-HLP-U
2mm Plugs with Solder Cups Set of 5 general purpose gold plugs, 2mm, with solder cups 1-HLP-0
Silver Wire Set of 5 Ag wires, 0.25mm diameter, 50mm long 1-HLA-005
Silicone Tubing for Silver Wire 1mm ID x 70mm Long Silicone Tubing 1-HLT-70
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assemblies 1-HLA-003
Adapter for BNC Holders to U-Type Headstages Connects BNC holders to CV and HS headstages with threaded collets (U-type) 1-HLB-U
Right-Angle Adapter for HL-U Electrode Holders Fits CV and HS headstages with threaded collets (U-type) 1-HLR-U

Model Cells

Description Details Part Number
Model Cell for Axoporator 800 Series Axoporator 800 model cell 1-SCE-1U

Number of Citations*: 82

Latest Citations:
For a complete list of citations, please click here.

 

Single-Cell Electroporation for In Vivo Imaging of Neuronal Morphology and Growth Dynamics

S Hossain, K PodgorskiK Haas - Neural Tracing Methods, 2015 - Springer
... We find that trains of short duration square pulses are effective, such as those produced
by common Grass Technologies SD9 stimulators or the Axoporator 800a from Molecular
Devices. An oscilloscope is useful to monitor pipette tip resistance. ...
 

In Vivo Study of Dynamics and Stability of Dendritic Spines on Olfactory Bulb Interneurons in Xenopus laevis Tadpoles

YB Huang, CR HuL Zhang, W Yin, B Hu - PloS one, 2015 - dx.plos.org
... interest. Dye was electroporated into neurons using 50 negative (positive for
dextran-conjugated dye) square 10–12 V pulses of 0.5 ms duration at a frequency
of 100 Hz generated by an Axoporator 800A (Axon Instruments, USA). ...
 
 

Single cell electroporation for longitudinal imaging of synaptic structure and function in the adult mouse neocortex in vivo

S Pagès, M Cane, J Randall, L Capello… - Frontiers in …, 2015 - ncbi.nlm.nih.gov
... For electroporation we used an Axoporator (800A) and a headstage (AP-1AX1MU, Molecular
Devices), attached to a micromanipulator (LN Junior, Luigs-Neumann) and positioned at an angle
of 30°. The target area was identified using the surface vascularization as a guide. ...
 

An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo

J Wietek, R Beltramo, M Scanziani, P Hegemann… - Scientific …, 2015 - ncbi.nlm.nih.gov
... ascorbate, 0.02 Alexa Fluor 594, and 10 HEPES (pH 7.2). An Axoporator 800A
(Molecular Devices) was used to deliver 50 hyperpolarizing pulses (−12 mV, 0.5
ms) at 50 Hz. At DIV 18–20, targeted patch-clamp recordings of ...
 
* as of November 13, 2015. Source: Google Scholar.  Search results include "Axoporator 800A" and "Axoporator Axon Instruments".