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Axopatch 200B Amplifier

For single-channel recording, whole-cell voltage- and current-clamp recordings, extracellular field potential recordings in ion channel, neuroscience, nanopore, voltammetry studies

The benchmark for single-channel recordings and other applications where an excellent signal-to-noise ratio is key.

Widely accepted as the gold-standard for ultra low-noise patch clamp recordings, the Axopatch™ 200B Capacitor Feedback Patch Clamp Amplifier is the premier microelectrode amplifier for ultra-low noise patch clamp recordings. Incorporates capacitor-feedback technology and active headstage cooling for single-channel recording, while resistive-feedback circuitry is used for two different ranges of whole-cell recording.
 
In current-clamp mode, offers two different speeds to optimize membrane potential recording, and allows correction for voltage errors due to pipette resistance. This makes the Axopatch 200B System the amplifier of choice for a scientist who performs basic research in the ion channel field and is the reason why researchers purchased thousands of these amplifiers from Molecular Devices over the past decade.
 
  • Actively cooled capacitor-feedback headstage reduces thermal noise for signal-to-noise ratios close to the theoretical limits of physics
  • Integrating headstage mode for measuring sub-picoamp currents to enable high-fidelity single-channel recordings from cell membranes and artificial bilayers
  • Resistive headstage mode for larger currents and noise levels in whole-cell recordings
  • Slim headstage design for easier access to the preparation on a crowded microscope stage
  • Series resistance compensation: Prediction helps achieve the desired command level faster by transiently supercharging the membrane potential
  • Series resistance compensation: Correction improves the bandwidth of the recording by eliminating the error introduced by the voltage drop across the series resistance
  • Dual-speed current clamp optimizes speed and stability through better matching of the clamp algorithm to experimental parameters
  • Integrated seal test in voltage- and current-clamp mode for easy monitoring of membrane and cell health parameters
  • Dual command potentials add flexibility by processing input signals from two different sources
  • Selectable holding command input with two internal range settings and an external input enable convenient control of the output waveform
  • Pipette capacitance compensation neutralizes the contribution of the glass pipette and the pipette holder to the capacitance of the circuitry
  • Cell capacitance compensation corrects for the capacitance of the cell membrane in whole-cell recordings
  • Selectable output gain with 10 settings to scale the output signal to the desired level
  • Leak subtraction for correction of leak currents without software-based algorithms
  • Sophisticated 4-pole Bessel low-pass filter acts as anti-aliasing filter and can be used to pre-condition the output signal
  • Configurable panel meter displays user-selectable parameters in real time for quick monitoring of the experiment
  • Zap facilitates patch rupturing by applying a voltage pulse for a user-defined duration
  • Two included pipette holders of different barrel lengths accept glass pipettes in most standard dimensions
  • User guide written by scientific consultants, with the assistance of Axon Instruments staff, for straightforward start-up and as an in-depth reference

Basic Ion Channel Research

Ion channels are pore proteins that are involved in a large variety of physiological processes. Basic research in the ion channel field furthers the understanding of ion channel function as well as their roles within cellular pathways and processes. Single-channel research helps with elucidating the mechanisms underlying ion channel function on a single-molecule level. Whole-cell and perforated patch clamp recordings are typically used to investigate the action of modulators on entire populations of ion channels in the membrane of a cell. Learn more.

Cell Pathway Analysis

Ion channels are involved in many cell pathways and understanding the function of ion channels in response to changes in membrane potential or the presence or absence of other molecules is important in order to understand exactly how ion channels participate in normal and abnormal biological processes such as cell differentiation and migration, disease states, neuronal communications, etc. Learn more.

Disease Research

Ion channels play a role in many diseases, including hypertension, cardiac arrhythmias, gastrointestinal, immune and neuromuscular disorders, pathological pain and cancer. By understanding the exact role that ion channels play in a particular disease, researchers might be able to find a way to affect the ion channel in such a way as to alter the course of the disease. Learn more.

Safety Assessment

A critical part of drug discovery involves safety assessments, such as hERG testing, in order to eliminate any compounds with potential safety concerns. Conventional patch clamp has played an important role in collecting evidence for the absence of side effects before submission of a drug candidate to regulatory authorities. Even if automated electrophysiology has shifted safety testing to earlier stages in the drug discovery process, follow-up with conventional patch clamp is an essential step in the workflow. Learn more.

In combination with a Digidata® 1550B Data Acquisition System and pCLAMP™ Software, the Axopatch 200B Amplifier forms the foundation of a patch clamp setup like those used in many electrophysiology labs around the world. Telegraphing between the software and the amplifier ensure correct scaling and logging of experimental parameters. The Clampex Data Acquisition Module, which is part of the pCLAMP Software, makes optimal use of the many advanced features of the Axopatch 200B Amplifier.

 

General Specifications

General Specifications
Dimensions (in.) 3.5 (H) x 19 (W) x 12.5 (D)
Dimensions (cm) 8.9 (H) x 48.3 (W) x 31.7 (D)
Weight 11.5 (5.1 kg)
Headstage (in.) 0.75 (H) x 0.70 (W) x 4.2 (D)
Headstage (cm) 1.8 (H) x 1.9 (W) x 10.5 (D)
Mounting plate (in.) 0.25 (H) x 2.0 (W) x 2.5 (D)
Mounting plate (cm) 0.6 (H) x 5.0 (W) x 6.2 (D)
Communications Analog and digital BNC
Rack use Standard 19" rack-mount (2U) with handles
Benchtop use Bayonet feet
Power
85-264 VAC (110-340 VDC)
50-60 Hz, 30 W (max)
Fuse 0.5 A slow (15 x 20 mm)
Line filter RFI filter included
Line cord Shielded line cord provided
Safety CE marking (Conformité Européenne)

Detail Specifications

Configurations

CV 203BU Headstage
 
Construction:
All critical components are in a sealed hybrid and cooled with a solid state cooling element
Configuration: High-speed, low-noise current to-voltage converter
Cooling: Input circuitry -15°C typical. Headstage cooling should be kept on at all times to ensure proper calibration of offset voltages
Gain (b):
1 mV/pA (b = 1) Patch or Whole-Cell modes
0.1 mV/pA (b = 0.1) Whole-Cell mode

 

 

 

 

 

 

 

 

Feedback Element:
Patch 1pF
Whole Cell β = 1, 500 MΩ in parallel with 1 pF
Whole Cell β = 0.1, 50 MΩ in parallel with 1 pF

 

 

 

 
 
 
 
Tuning (Whole Cell mode only): Tuning circuit to idealize response of the feedback resistor is contained in the main instrument. Tuning is automatically bypassed when the capacitive feedback is selected.
 
Pipette-capacitance-compensation injection capacitor: 1 pF
 
Whole-Cell-Capacitance-Compensation Ranges:
Patch none
Whole Cell β = 1 : 0.3-100 pF
Whole Cell β = 0.1 : 3-1000 pF

 

 

 

 

 

  • Case: Connected to ground. Case jack mates to 1 mm plugs.
  • Bandwidth: Test signal applied via Speed Test input
    • Internal:
      • (Patch mode): Internal: 140 kHz
      • 70 kHz whole-cell mode
    • External:
      • 100 kHz (limited by Bessel filter)
  • Capacitative load stability: 1000 pF, 0 Ω in series
  • Maximum instrument noise: Measured with minimal external noise sources ( i.e. radiated line-frequency noise, mechanical vibration) 8-pole Bessel filter.
Max. Instrument Noise: With 1-HL-U Holder
  Patch Whole Cell Whole Cell
Line Frequency and Harmonic β = 1
0.005 pAp-p
β = 1
0.005 pAp-p
β = 0.1
0.005 pAp-p
0.1 - 100 Hz 0.030 pAp-p 0.50 pAp-p 1.6 pAp-p
0.1 - 1 kHz 0.015 pArms 0.50 pArms 0.75 pArms
0.1 - 5 kHz 0.060 pArms 0.65 pArms 1.65 pArms
0.1 - 10 kHz 0.130 pArms 1.10 pArms 3.0 pArms
Max. Instrument Noise: Without Holder
  Patch Whole Cell Whole Cell
Line Frequency and Harmonic β = 1
0.005 pAp-p
β = 1
0.005 pAp-p
β = 0.1
0.005 pAp-p
0.1 - 10 kHz 0.145 pArms 1.10 pArms 3.0 pArms

 

Headstages

Description Details Part Number
Axoclamp 900A Headstage HS-9A X0.1U x0.1 headstage 1-2950-0359
Axoclamp 900A Headstage HS-9A X1U x1 headstage 1-2950-0360
Axoclamp 900A Headstage HS-9A X10U x10 headstage 1-2950-0361
Axoclamp 900A Headstage VG-9A X10U x10 virtual ground headstage 1-2950-0362
Axoclamp 900A Headstage VG-9A X100U x100 virtual ground headstage 1-2950-0363
MultiClamp 700B Headstage CV-7B Patch-clamp headstage 1-CV-7B
MultiClamp 700B Headstage CV-7B/BL Bilayer headstage 1-CV-7B/BL
MultiClamp 700B Headstage CV-7B/EC Electrochemistry headstage 1-CV-7B/EC

Electrode holders, adapters, and holder components

Description Details Part Number
Electrode Holder for U-Type Headstages Fits glass pipettes with outer diameter of 1.0 - 1.7 mm 1-HL-U
Electrode Holder Replacement Caps Set of 2 polycarbonate caps for HL-U holders 1-HL-CAP
Cone Washers 1.1mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.0 - 1.1 mm 1-HLC-11
Cone Washers 1.3mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.1 - 1.3 mm 1-HLC-13
Cone Washers 1.5mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.3 - 1.5 mm 1-HLC-15
Cone Washers 1.7mm ID Set of 10 orange cone washers for HL-U holders, fit glass with outer diameter of 1.5 - 1.7 mm 1-HLC-17
Pins 1mm for HL-U Holders Set of 3 brass pins for HL-U holders, 1mm 1-HLP-U
2mm Plugs with Solder Cups Set of 5 general purpose gold plugs, 2mm, with solder cups 1-HLP-0
Silver Wire Set of 5 Ag wires, 0.25mm diameter, 50mm long 1-HLA-005
Silicone Tubing for Silver Wire 1mm ID x 70mm Long Silicone Tubing 1-HLT-70
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assemblies 1-HLA-003
Adapter For BNC Holders To U-Type Headstages Connects BNC holders to CV and HS headstages with threaded collets (U-type) 1-HLB-U
Right-Angle Adapter for HL-U Electrode Holders Fits CV and HS headstages with threaded collets (U-type) 1-HLR-U

Model Cells

Description Details Part Number
Model Cell for Oocytes Axoclamp/ GeneClamp model cell for oocytes. Connects to U-type HS series headstages 1-MCO-2U
Model Cell for TEVC / DSEVC Axoclamp/ GeneClamp model cell for two-electrode voltage clamp/ discontinuous single-electrode voltage-clamp conditions. Connects to U-type HS series headstages 1-CLAMP-1U
Model Cell for Whole Cell/ Single Channels Axopatch/ GeneClamp/ MultiClamp model cell for whole-cell / single-channel patch-clamp conditions. Connects to U-type CV series headstages 1-PATCH-1U
Model Cell for Bilayers Axopatch/ GeneClamp/ MultiClamp model cell for bilayer conditions. Connects to U-type CV series headstages 1-MCB-1U

Cables

Description Details Part Number
Cable To Connect Axoclamp 2 Headstages To Axoclamp 900A Amplifier Allows Axoclamp 2 headstages (HS-2, VG-2) to be used on Axoclamp 900A Amplifiers 1-2100-0934

Miscellaneous

Description Details Part Number
SoftPanel Amplifier Control Unit Provides physical knob and button control for computer-controlled Axoclamp 900-series and MultiClamp 700-series amplifiers. Requires a USB connection. 1-SOFTPANEL (USB)
Remote Buzz For Axoclamp 900A Hand-held buzz duration control for Axoclamp 900A Amplifier (1-50ms) 1-2950-0366
Silver/Silver Chloride Pellet Assemblies Set of 3 Ag/AgCl pellet assembly and Ag wire 1-HLA-003

Number of Citations*: 26,200

Latest Citations:

For a complete list of citations, please click here.

Ion Selectivity of Lysenin Channels

J May, S Bryant, M Johnson, R Good - 2015 - scholarworks.boisestate.edu
... The solutions bathing the membrane are wired to an Axopatch 200B electrophysiology
amplifier through Ag/AgCl electrodes. ... The solutions bathing the membrane are wired to an
Axopatch 200B electrophysiology amplifier through Ag/AgCl electrodes. ...
 
 

Actions of Bupivacaine, a Widely Used Local Anesthetic, on NMDA Receptor Responses

MA Paganelli, GK Popescu - The Journal of Neuroscience, 2015 - Soc Neuroscience
... intracellular Mg 2+ block. Currents were amplified and filtered at 2 kHz (Axopatch
200B), sampled at 5 kHz (Digidata 1440A) and stored as digital files using
pClamp10.2 software (Molecular Devices). Macroscopic current peak ...

Chemical activation of the mechanotransduction channel Piezo1

R Syeda, J Xu, AE Dubin, B Coste, J Mathur, T Huynh… - Elife, 2015 - elifesciences.org
... Stretch-activated currents were recorded using 244 Axopatch 200B amplifier (Molecular Devices
Axopatch 200B). ... ratio. Electrode carrying the proteoliposome droplet was connected to the working
end of the 276 amplifier head-stage (Molecular Devices Axopatch 200B). ...
 

Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

C Weichbrodt, H Bajaj, G Baaken, J Wang, S Guinot… - Analyst, 2015 - pubs.rsc.org
... Planar lipid bilayer assays (BLM). The ion current was recorded using an Axopatch
200B (Axon instruments) amplifier and the signal was filtered by using a low-pass
4-pole Bessel filter at 10 kHz and sampled at a frequency of 50 kHz. ...

Murine and human CFTR exhibit different sensitivities to CFTR potentiators

G Cui, NA McCarty - … Journal of Physiology-Lung Cellular and …, 2015 - Am Physiological Soc
... and 127.6 U/ml PKA (pH 7.5). CFTR currents were measured with an Axopatch 200B
amplifier (Molecular Devices; Sunnyvale, CA), and were recorded at 10 kHz to DAT
tape. For subsequent analysis, records were played back ...
 

Inactivation of Endothelial Small/Intermediate Conductance of Calcium‐Activated Potassium Channels Contributes to Coronary Arteriolar Dysfunction in Diabetic …

Y Liu, A Xie, AK Singh, A Ehsan… - Journal of the …, 2015 - Am Heart Assoc
... Perforated whole‐cell voltage‐clamp using Axopatch200B amplifier (Molecular Devices, Foster
City, USA) was employed for recording K + currents. ... Perforated whole‐cell current‐clamps were
used to record membrane potentials (Axopatch200B amplifier, Molecular Devices). ...
 

The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current

O Revah, L Libman, IA Fleidervish, MJ Gutnick - PloS one, 2015 - dx.plos.org
... microscope (Carl Zeiss, Oberkochen, Germany). Currents were recorded in
whole-cell configuration using an Axopatch 200A or Axopatch 200B amplifier
(Molecular Devices, Foster City, CA). Patch pipettes were manufactured ...
 
 

GABAergic interneuronal loss and reduced inhibitory synaptic transmission in the hippocampal CA1 region after mild traumatic brain injury

CP Almeida-Suhett, EM Prager, V Pidoplichko… - Experimental …, 2015 - Elsevier
Patients that suffer mild traumatic brain injuries (mTBI) often develop cognitive impairments,
including memory and learning deficits. The hippocampus shows ah.
 

Functional characterization of rare variants implicated in susceptibility to lone atrial fibrillation

K Hayashi, T Konno, H Tada, S Tani, L Liu… - Circulation: …, 2015 - Am Heart Assoc
... Potassium or sodium currents were studied using the whole-cell patch clamp technique with
an amplifier, Axopatch-200B (Molecular Devices, Sunnyvale, CA), ... Two-tailed patch clamp
technique with an amplifier, Axopatch-200B (Molecular Devices, Suununnynyn vale, CA CA), ...

 

* as of November 13, 2015. Source: Google Scholar.  Search results include "Axopatch 200B" and "Axopatch Axon Instruments".